ECL stands for Enhanced Chemiluminescence. As you probably know, we use it to detect and quantify specific proteins on a membrane. The chemicals in ECL, when mixed together, react to produce light. This reaction is catalyzed by the enzyme horseradish peroxidase. In a Western blot, the secondary...
Voronoï tessellation analysis was computed to quantify cluster size using SR-Tesseler63,64, and for co-localization analysis Spearman’s rank correlation and Manders’ coefficients were computed using Coloc-Tesseler63,64. Segmentation of the clusters and Manders’ coefficients were obtained by ...
9. Prepare a protein standard curve using the Bradford method to determine protein concentration. Dilute the total protein at least 10-fold before measurement to reduce the interference of detergents in the cell lysis buffer (quantify and aliquot, can be stored at -20°C for one month). ...
How is phosphorylated protein detected in Western blot? To quantify a phosphorylated protein,fluorescent conjugated antibodiescan be used to detect phosphorylated and total proteins on the same blot in a process called multiplexing. How is tyrosine phosphorylation detected? The development of monoclonal an...
HPLC was used to quantify the cellular levels of ATP and NAD+ as reported previously (29, 30). Nucleotides were extracted by perchloric acid precipitation (31), and solvent A (75 μl; 0.1 m potassium phosphate and 4 mm tetrabutylammonium bisulfate (pH 6.0), diluted 64:36 in water (v/v...
What is the difference between an observation and an inference? Why is it important to quantify your data? Does a test have to be an experiment? How are qualitative and quantitative observations the same? How are they different? What is the difference between ...
Therefore, the development of tools and methods to detect and quantify α-syn oligomers has become increasingly crucial for mechanistic studies to understand their role in PD, and to develop new diagnostic methods and therapies for PD and other synucleinopathies. The majority of these tools and ...
CD95/Fas is an apoptosis inducing death receptor. However, it also has multiple nonapoptotic activities that are tumorigenic. Chronic stimulation of CD95 on breast cancer cells can increase their cancer initiating capacity through activation of a type I
Native connexin hemichannel Po is significantly reduced when [Ca2+]o is elevated to physiological levels, e.g. 2 mM. Macroscopic current–voltage relationships (e.g. −80 to +80 mV) and differential high–low [Ca2+]o whole cell current measurements are routine ways to quantify putative ...
Nelson J, Bishay F, Van Roodselaar A, Ikonomou M, Law FC (2007) The use of in vitro bioassays to quantify endocrine disrupting chemicals in municipal wastewater treatment plant effluents. Sci Total Environ 374:80–90 CAS Google Scholar NGS (2016) Natural gas from shale hydraulic fracturing...