Western Blot Troubleshooting: Weak/No Signal & Other Western Blot ppt Western Blot Video ProtocolDownload PDF version Obtaining perfect, publication-ready results straightaway is not exactly the norm when performing Western blotting. However, there are steps you can take to refine your blotting exper...
Western blot is an indispensable mechanism in the modern biomedical research laboratory, as well as in laboratories doing research in other areas. It is considered as an analytical technique used mainly in molecular biology and immunogenetics where antibodies are used to specifically detect their antigen...
How To : Run a Western BlotBiologicals, Novus
What protein should I use to block a transfer membrane for a western blot? My name is Srikanth, I'm a Product Scientist at Cell Signaling Technology, and this is CST Tech Tips. In regards to western blotting, a common question we always get is, what should I use, milk or BSA to ...
Flexibility –The same secondary antibody can be used to detect multiple targets by pairing with multiple primary antibodies. More versatile –The same primary antibody can be paired with various secondary antibodies depending on the intended application (e.g., HRP conjugates for western blot and flu...
How to avoid cross-reactivity in western blotIf possible, it is recommended to use the secondary antibody specific to the first antibody used; for example, if the primary antibody is mouse, then the secondary antibody should be anti-mouse IgG-HRP. In case the secondary antibody is polyclonal ...
How Does the Western Blot Test Work? Western blots tests are also known as protein immunoblots. These tests are used to detect specific proteins in a sample. The basic technique of a Western blot involves sorting proteins by length on a gel. Then that grid is probed with antibodies that re...
we have begun putting our own antibodies to the test. We picked a handful of Santa Cruz’s most cited antibodies and performed head-to-head comparisons on multiple applications ranging from western blot to flow cytometry. Our decision to test Santa Cruz’s most cited antibodies was risky, but...
Proteintech's collection of technical tips will help you optimize extraction during lysate preparation.1. Cell lysis For suspension cell culture samples, cells can be collected directly by centrifugation and washed with PBS or with saline 2–3 times to
1.Evaluate Cre mRNA transcripts via RT-PCR or Northern blot using mRNA derived from the target tissue. 2.Evaluate Cre protein using IHC or Western blot. A Cre antibody applicable to both IHC and WB is recommended. Keep in mind that appropriate fixation conditions is required for cytosolic prot...