To know how to analyze western blot data, Look for the sizes of the bands. These will be represented by a number, either followed by “kDa” or preceded by “p.” This is the size of the protein which has been detected and is the scale on which the proteins are separated in a Wes...
it allows researchers to analyze proteins in their natural or “native” state. This may be desirable when analyzing bound proteins or complexes, for example, when it is important their biological activity remains intact. As the aim here is to preserve the natural...
Materials and methods: The cells were exposed to various intensities and durations of 60-Hz sinusoidal MF in combination with various serum concentrations in the media. To analyze MF effects on cell growth, cell counting, trypan blue exclusion assay, Western blot analysis, flow cytometry, enzyme-...
Standard curves are used in various assays, such as ELISA, western blot, and PCR, to quantify the amount of a target molecule in a sample. They are generated using a set of known standards, usually in the form of a series of dilutions of a stock solution, and a calibration curve is ...
How to analyze autosomal dominant mutations? a. How does the Kruppel gap gene work genetically and molecularly during segmentation in drosophila? b. Why do mutations in this gene give such a phenotype? Discuss what conclusions can be drawn from the pedigree eg assumin...
How does RNA polymerase choose its DNA strand to transcribe? What information would you get from a western blot that you would not get from a northern blot when used to analyze the expression of the same gene? How do geneticists determine the function of a...
DNA microarray technology is a powerful technique that was recently developed in order to analyze thousands of genes in a short time. Presently, microarrays, or chips, of the cDNA type and oligonucleotide type are available from several sources. The number of publications in this area is increasin...
In this simulation, you will learn how tumor cells send signals to surrounding cells to help promote tumor growth, and how this signal is transmitted inside the cell. Analyze patient samples by western blotting As a researcher in the R&D department of a big pharma company, your mission will ...
To analyze the amount of DDB complex formation by size exclusion chromatography (SEC), 500 nM fully purified dynein, 1 μM pig dynactin and 4 μM SNAPf-BICD2N (dimer) were mixed in a volume of 80 μL and incubated on ice for 15 min before SEC was carried out as described above. ...
Molecular biology Most life-sciences laboratories utilize molecular biology techniques, although not all will be used for any one experiment. It is critical to understand what results are needed, and what techniques will give that data. Often there...