soak t he gels in the dye. to reduce background and make the bands easier to visualize, elude the extra stain with a solvent; this step is called destaining. destaining can
Here, we used STORM super-resolution microscopy and single-particle-tracking (SPT) experiments to analyze the differential cell membrane distribution and diffusion behavior of PS, ecto-CRT, and CD47 on adherent HeLa cells. We compared viable cells to cells in early apoptotic stages induced by UVB...
Materials and methods: The cells were exposed to various intensities and durations of 60-Hz sinusoidal MF in combination with various serum concentrations in the media. To analyze MF effects on cell growth, cell counting, trypan blue exclusion assay, Western blot analysis, flow cytometry, enzyme-...
In this simulation, you will learn how tumor cells send signals to surrounding cells to help promote tumor growth, and how this signal is transmitted inside the cell. Analyze patient samples by western blotting As a researcher in the R&D department of a big pharma company, your mission will ...
if you cannot produce working results, the inclusion of methods and perspectives from other disciplines can help you define, grasp and analyze your subject much better and stimulate ideas. See page 44 and page 84 for interdisciplinary work. Sometimes, the merging of different domains can lead to...
DNA microarray technology is a powerful technique that was recently developed in order to analyze thousands of genes in a short time. Presently, microarrays, or chips, of the cDNA type and oligonucleotide type are available from several sources. The number of publications in this area is increasin...
Materials and methods: The cells were exposed to various intensities and durations of 60-Hz sinusoidal MF in combination with various serum concentrations in the media. To analyze MF effects on cell growth, cell counting, trypan blue exclusion assay, Western blot analysis, flow cytometry, enzyme-...
How to open an SCN file You can open SCN files in Microsoft Age of Mythology, Age of Empires, and Age of Empires II (Windows) and use them as in-game scenarios. To open an SCN file, you must place the file in your game'sScenariodirectory. Then, you will be able to select the ...
Staals continued: “We want to work with fluorescent and chemi Westerns and need a system which can analyze a whole range of different dye types. We looked at two CCD-based imagers and decided on the G:BOX Chemi because the...
Hormone metabolism can vary from one species to another. In humans, specific UDP-glucuronosyltransferase (UGT) enzymes transform androgens (the male hormones) into glucuronide derivatives, which are easier to eliminate. Whether a similar mechanism also takes place in mice has never been ascertained....