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How To : Run a Western BlotBiologicals, Novus
If the Western blot shows high background signal, revising the washing steps on the next run-through has proven to be useful. Increasing washing times and volumes will reduce background signal along with performing additional buffer changes or using a stronger detergent (e.g., SDS instead of ...
A loading control is a protein that is highly and constitutively expressed in your sample and that is used as a positive control in your western blot experiment. The most important reason for using a loading control is to ensure the reliability of your data and conclusions, as it helps demons...
What protein should I use to block a transfer membrane for a western blot? My name is Srikanth, I'm a Product Scientist at Cell Signaling Technology, and this is CST Tech Tips. In regards to western blotting, a common question we always get is, what should I use, milk or BSA to bl...
Solution:A more efficient and accurate alternative to HKPs exist for western blot normalization. Researchers can normalize western blots using the total protein in the sample, known as total protein normalization (TPN). In TPN, the abundance of the target protein is normalized to the total amount...
A further important difference between in-cell Westerns and traditional Western blotting (using enzyme-labeled antibodies and chemiluminescent detection) is that in-cell Westerns have a much higher throughput. This is because they eliminate the need to perform polyacrylamide gel electrophoresis (PAGE) ...
2. Perform the procedure at a low temperature. For the preparation of protein samples of common mammalian tissues or cells, all steps can be performed at low temperature, and all reagents should be precooled to reduce protease activity and prevent protein degradation. In particular, digestive syst...
Almost everyone who is HIV-positive has detectable levels of antibodies at 3 months. So if you test negative a month or two after your exposure, your doctor may want you to retest after 3 months to be sure. Post-exposure prophylaxis ...
Hanjin has stopped the container on rail, and has chosen to hold all the containers to perform a customs inspection on another container. We can not do business like this any more. I am not sure why Hanjin and Translink has started to operate this way, but 应该的这个容器ws提供下个月...