In a Western blot, the secondary antibody is conjugated to horseradish peroxidase. And because the secondary antibody binds specifically to a primary antibody that binds specifically to a target protein, the emission of light from ECL is proportionate to the amount of that target protein. What Are...
A micro BCA assay was used to quantify protein concentration. Cell lysates were precleared with dynabeads protein G magnetic beads (Thermofisher, 10003D). Immunoprecipitation was performed overnight at 4 °C with 40 µl of dynabeads protein G that were preincubated with 8 µL of a...
How is phosphorylated protein detected in Western blot? To quantify a phosphorylated protein,fluorescent conjugated antibodiescan be used to detect phosphorylated and total proteins on the same blot in a process called multiplexing. How is tyrosine phosphorylation detected? The development of monoclonal an...
CD95/Fas is an apoptosis inducing death receptor. However, it also has multiple nonapoptotic activities that are tumorigenic. Chronic stimulation of CD95 on breast cancer cells can increase their cancer initiating capacity through activation of a type I
Therefore, the development of tools and methods to detect and quantify α-syn oligomers has become increasingly crucial for mechanistic studies to understand their role in PD, and to develop new diagnostic methods and therapies for PD and other synucleinopathies. The majority of these tools and ...
Western Blot Analysis Equal amounts of protein from cell lysates were resolved by reducing SDS-PAGE and transferred to nitrocellulose membranes. Proteins were detected using primary antibodies at a 1:1000 dilution: phospho-AMPK (Thr-172), phospho-acetyl-CoA carboxylase (phospho-ACC), phospho-eIF2...
What is the difference between an observation and an inference? Why is it important to quantify your data? Does a test have to be an experiment? How are qualitative and quantitative observations the same? How are they different? What is the difference between ...
Native connexin hemichannel Po is significantly reduced when [Ca2+]o is elevated to physiological levels, e.g. 2 mM. Macroscopic current–voltage relationships (e.g. −80 to +80 mV) and differential high–low [Ca2+]o whole cell current measurements are routine ways to quantify putative ...