[工具]Western Blot量化,蛋白质印迹量化 今天给大家讲讲如何用ImageJ进行Western Blot的量化。这个量化的方法是参照由Hossein Davarinejad 写的Quantifications of Western Blots with ImageJ。 用photoshop将你WB的图片转成Graystyle模式,保存为JPEG格式。(这一步很重要) 打开ImageJ, 找到Analyze --> Set Measurements...
With Image Studio, you get flexibility and essential quantification results for a variety of assays. Tools for image acquisition, analysis, and display adjustment are all organized into ribbons, so the function you need is never more than a few clicks away. ...
Given the complexity of quantifying within a linear range the multiple APOE bands of differing intensity detected by Western blot analysis, we used a human APOE sandwich ELISA that would allow for better quantification of blood plasma APOE levels. One-way ANOVA of ELISA on 12-month-old mice ...
Cerebellum western-blot and quantification of GRP78 protein levels in 30 week-old WT, VPA-treated and non-treated CMVMJD135 mice.Sofia, EstevesSara, DuarteSilvaLuana, NaiaAndreia, NevesCarvalhoAndreia, TeixeiraCastroAna, Cristina RegoAnabela, SilvaFernan...
nstitutes of Health Western Blot Analysis : Protein Quantification National Institutes of HealthWestern Blot Analysis : Protein Quantification National Institutes of HealthGenomics, CancerBranch, Genetics
SH2 (1:10 or 1:100 dilution). Grb2-SH2 binding to p56 and p60 was readily detectable with Wes, indicating specific tyrosine phosphorylation of both proteins. (C) Quantification of signal peaks in the Grb2-SH2 Wes lane view (orange box in B) and electrograph view (or...
Right, quantification of the TP signal. Square root transformed TP amount (x axis) and sum total normalized, square root transformed integrated density (y axis) are plotted in a line-connected XY scatter graph. Data are shown as the mean ± SD for triplicate experiments using separate pooled...
Two common software packages for Western blot quantification and analysis are Image Studio Software and Empiria Studio Software. Journals require that your images were not manipulated and that raw data were not compromised in a way that positively or negatively affects your conclusions. As a ...
The combination of molecular weight resolution, sensitivity and signal linearity on an automated platform enables the rapid quantification of hundreds of specific proteins and protein modifications in complex samples. This high-throughput western blot approach allowed us to identify and characterize ...
The FUSION FX Spectra is a western blot and chemiluminescence imaging system with flexible upgrade paths. It is designed for molecular biology laboratories.