What is a GFP(Green Fluorescent Protein)? How might researchers use a GFP-labeled protein to study cancer? Fluorescence: Fluorescence is a certain substance that emits visible or invisible radiation. Fluorescence is one type of luminescence. It has many practical ...
Many fluorescent proteins have a tendency to oligomerize and this can result in aggregation and in cases where your protein of interest also oligomerizes (such as actin or tubulin) oligomerisation of FPs can inhibit correct formation of oligomers. Luckily there are mutants of GFP and GFP-derived...
Where the protein locates determines its interaction partners and thus its functions. Cells sort proteins using a series of encoded signal peptides (or localization signals) within the protein, analogous to postcodes, that determine where the protein should be transported e.g., to the...
a可不可以对你倾尽所有温柔。 Can lean completely all to you gentle.[translate] a你不听流行音乐,对吗? You do not listen to the pop music, to?[translate] aThe enabling technology is the expression of a fusion of GFP (or any fluorescent protein) with a sequence-specific RNA binding ...
The use of in vivo two-photon imaging, involving the assessment of layer 5 neuron GFP-labeled dendrites and axons, has provided a new means of longitudinal circuit assessment. A series of in vivo studies that were focused on cortex (Zhang et al., 2005) demonstrated dynamic changes to ...
1). The first tag is a fusion protein containing a strong antigenic region (such as Protein G or GFP) fused to a separate smaller tag (such as streptavidin or calmodulin-binding peptide). The two tags are usually linked by a specific cleavage site (such as the Tobacco Etch Virus or ...
When these cells are ectopically transplanted into a recipient animal, they give rise to tumors that consist of three germ layer-derived cells. EC cells were then shown to have the ability to contribute to host embryo development to make a chimeric animal when injected into blastocyst-stage ...
Besides, CaMBL1 was localized at the plasma membrane after transient expression of a CaMBL1-Green Fluorescent Protein (GFP) fusion protein in onion epidermal cells [56]. Thus, LecPs, LecRLPs and LecRLKs are readily found at the cell surface. LecRLKs have been shown to dimerize upon ...
Recently MEIS1 emerged as a major determinant of the MLL-r leukemic phenotype. The latest and most efficient drugs effectively decrease the levels of MEIS1 in cancer cells. Together with an overview of the latest drugs developed to target MEIS1 in MLL-r
Accordingly, the protein fragment binds liposomes in membrane floatation assays. In human U2OS cells, a fusion protein where the Nup133 ALPS motif was introduced into EGFP localizes to tubular ER structures and small liposomes, both indicating a preference for highly curved membranes [47,48]. ...