In fusion gene IV (gr), gfph replaced gfp2 with a linker of nine amino acids. The fusion gene cassettes I and III were placed into pBluescript KS II (+) and the fusion gene II and IV into mammalian expression vector pCEP4. The above plasmids were transformed into E. coli , ...
Nluc is a bioluminescent protein that emits 450 nm luminescence by adding the substrate “Furimazine” (18) and can be utilized as an efficient donor of BRET (17). The conjugated protein consists of EGFPpHΔC12 (EGFPpH lacking C-terminal 12 amino acids), the linker amino acid sequence of ...
Yeast DHODH and Aequorea GFPmut2 (1) coding sequences were inserted into an E. coli-Plasmodium falciparum shuttle plasmid (pHHMC*/3R0.5) (2), such that DHODH-GFP were fused into a single coding sequence (including a 10Ala-1Gly linker segment). ...
Extending the extracellular linker of GRAPHIC is a potential strategy to overcome the variable distance between cells, although this strategy may not be effective in overcoming the problem of the high mobility of cancer cells. sGRAPHIC successfully overcomes the shortcomings of GRAPHIC, and expands the...
However, recent biophysical studies showed that the fusion of GFP to full length Tau (Tau_FL-GFP) or the short repeat domain Tau containing a pro-aggregating mutation (Tau_RDΔK-GFP), separated with a 13–14 amino acids linker, significantly alters the β-strands packing within the fibrils...
. N-terminally tagged mEGFP-Drp1 and C-terminally tagged Drp1-mEGFP were overexpressed inE. colifor recombinant protein production and biochemical and biophysical characterization in vitro, and in MEFs for examining Drp1 quaternary structure, oligomerization dynamics, and function in vivo. Linker sequence...
然后通过融合 PCR 技术将 Gluc 基因与 G11 基因通过 Linker 连接并插入到 pcDNA3.1 上,与 G1-10 表达质 粒共转染 293T 细胞,测定细胞上清中 Gluc 荧光素酶的活性,并进行 GFP 荧光 观察。为了更方便对病毒进行细胞水平的观测和筛选,利用慢病毒表达系统构建 能稳定表达 G1-10 的 MDCK 细胞系,再向细胞系中...
Fusion of the binding domain of Raf-1 kinase with green fluorescent protein for activated Ras detection by fluorescence correlation spectroscopy. Ras proto-oncogenes play a central role in cell proliferation by the regulation of signal transduction pathways from receptors of the outer cell membrane t...
Genomic walking PCRs and synthetic DNA linker assemblage were carried out according to the method described by Morris et al, Appl Environ Microbiol (1995) 61:2262-2269. The PENTGWF and GFPGWR primers were used in combination with primers complementary to the generic linker for GWPCR reactions...
TGL is produced from the expression of a triple-fusion reporter gene (TGL gene) in which transcription and translation are processed through a single open reading frame. TGL consists of three fused protein subunits: HSV1-TK, GFP, and Fluc, where polylysine is used as a linker to connect ...