Computational investigations showed that the dual-purpose sequence was structurally stable and may be a good choice to use as both a linker and a fluorescence marker between two moieties in a fusion protein.Rezaie, EhsanBaqiyatallah Univ Med SciMohammadi, Mozafar...
The fusion protein linker 的选择 蛋白融合Linker的设计与选择 接头序列(Linker)设计是基因融合技术能否成功的关键技术之一。即通过一段适当的核苷酸序列将不同的目的基因连接起来, 使其在适当的生物体内表达成为一条单一的肽链, 其中起连接作用的氨基酸称为Linker[1]。融合蛋白中的两种成分能否分别形成正确的空间结构、...
35、pastorisj. protein eng, 2001, 14(9): 711-715.2 chiquito jc, feng ja. linker: a program to generate linker sequences for fusion proteinsj. protein eng, 2000, 13(5): 309-312.3 ryoichi a, hiroshi u, atsushi k, et al. design of linkers which effectively separate domains of a bifu...
Stable linker peptides for a cellulose binding domain lipase fusion protein expressed in Pichia Pastoris[J]。 Protein Eng, 2001, 14(9): 711-715. [2] Chiquito JC, Feng JA. LINKER: a program to generate linker sequences for fusion proteins[J]。Protein Eng, 2000, 13(5): 309—312。ﻫ...
蛋白融合Linker的设计与选择接头序列Linker设计是基因融合技术能否成功的关键技术之一,即通过一段适当的核苷酸序列将不同的目的基因连接起来,使其在适当的生物体内表达成为一条单一的肽链,其中起连接作用的氨基酸称为Linker1,融
Bivalent, bispecific single-chain antibody fusion protein construction appears to be a promising tool for tumor therapy. One of its drawbacks is that the function and activity of the fusion proteins have varied affinity and/or anti-tumor activity compared with the original molecules from which they...
However, when we changed only two nucleotides within the 2346 nucleotide gene to eliminate two over-represented or “slow” codon pairs, one within each linker, without altering the amino acid sequence of the fusion protein we were able to achieve expression. The increased protein expression did ...
Here, we have used our cell free antigen processing system to examine epitope hierarchy under two different conditions; a) among two different protein antigens whose immunodominant epitopes are known, and b) in single recombinant proteins that included spacer/affinity tag sequences for ease of purif...
e Micrographs of HeLa cells expressing a Tom20-GFP-DIAPH1-CT fusion protein that contains the linker sub-domain of DIAPH3 (green), fixed and stained for β- or γ-actin (magenta). f Micrographs of HeLa cells expressing a Tom20-GFP-DIAPH3-CT fusion protein containing the linker sub-...
A preferred embodiment of the peptide linker of the invention comprises the sequence: GSTSGSGXPGSGEGSTKG (SEQ. ID NO 1), where X is a charged amino acid, preferably lysine or arginine. Methods of making linked fusion polypeptides using the peptide linker of the invention are claimed. DNA ...