GFP IN MOTION: PHOTOCHEMISTRY: Ultrafast method reveals how green fluorescent protein changes during proton transferdoi:10.1021/cen-v087n046.p006aCelia ArnaudAmerican Chemical Society
Once a cell can be seen, it can be studied and manipulated. The story of the discovery and development of GFP also provides a very nice example of how scientific progress is often made: through accidental discoveries, the willingness to ignore previous assumptions and take chances, and the ...
A Tsinghua team traced the production of a gas warning other plants of aphid attack to a gene called NAC2. They also showed that plants that lack this gene are more susceptible to viruses. Here the scientists examined the ...
Adding a nuclear localisation signal (NLS) to virtually any protein would result in protein re-routing to the cell nucleus. The reverse process is also possible, using nuclear export signal (NES) to export a protein out of the nucleus. Having both NLS/NES will result in the pr...
Cropped image showing GFP expression in onion cells. Learn faster. Dig deeper. See farther. Join the O'Reilly online learning platform. Get a free trial today and find answers on the fly, or master something new and useful. Learn more ...
Myosin VI (Myo6) is the only minus-end directed nanomotor on actin, allowing it to uniquely contribute to numerous cellular functions. As for other nanomotors, the proper functioning of Myo6 relies on precise spatiotemporal control of motor activity via
This process is catalyzed by the viral HA protein.14 When exposed to the acidic environment of the endosome, HA, whose each terminus is inserted into each membrane, undergoes a conformational change that bends two α-helical stretches and generates a driving force for membrane fusion. These ...
UnveilingHow an Archetypal Fluorescent Protein Operates:Theoretical Perspective on the Ultrafast Excited State Dynamics ofGFP Variant S65T/H148D.Greenfluorescent protein variant S65T/H148D has been reportedto host a photocycle involving the photoinduced proton transfer reactionbetween the chromophore and ...
GFP tagging of budding yeast chromosomes reveals that protein–protein interactions can mediate sister chromatid cohesion Background Precise control of sister chromatid separation is essential for the accurate transmission of genetic information. Sister chromatids must remain linked to each other from the ...
结论 建立了以 ZMBJ-CMV 载体诱导的忍冬基因沉默体系,农杆菌 A 值为 0.8,注射后再进行真空浸润,能够提高忍冬 目标基因的沉默效率,为开展忍冬关键基因的功能研究提供了技术支撑. 关键词:忍冬;LjPDS;VIGS;叶绿素;类胡萝卜素;沉默体系 中图分类号:R286.12 文献标志码:A 文章编号:0253 - 2670(2024)04 - 1309 -...