This paper presents a global statistical analysis of the RNA-Seq results of the entire Mus musculus genome. We explain aging by a gradual redistribution of limited resources between two major tasks of the organism: its self-sustenance based on the function of the housek...
RNA-sequencing is a process used to quantify the amount of RNA and read the sequences present in a biological sample. RNA-Seq has many applications and can be used to understand which genes are expressed during different developmental time-points or in response to stress.Answer ...
* Automated library preparation of any 2-pool Ion AmpliSeq panel on the Ion Chef System. To find out more about NGS, see theNGS Basics articles. A note about fragment analysis Capillary electrophoresis instrumentsare capable of performing both Sanger sequencing a...
Stem cells derived from U-78 cell lines were found to produce high levels of m-RNA of the gene encoding calcium activated K + channel [29]. It has been reported that TRAM-34, a specific blocker of intermediate Ca2+-activated K + channel, diminishes tumor infiltration of GL-15 GBM cells...
This study uses single-cell RNA-seq to characterize different subsets of mouse thymic NKT cells. CAS PubMed PubMed Central Google Scholar Wang, H. & Hogquist, K. A. CCR7 defines a precursor for murine iNKT cells in thymus and periphery. eLife 7, e34793 (2018). PubMed PubMed Central...
In this tutorial, we first analyze one paired-end RNA-Seq data set from a single dendritic cell sequenced 6 hours after lipopolysaccharide (LPS) stimulation. Then we demonstrate how to perform differential expression analysis on two groups of single cells. Lastly, we show how we can use the ...
In this tutorial, we first analyze one paired-end RNA-Seq data set from a single dendritic cell sequenced 6 hours after lipopolysaccharide (LPS) stimulation. Then we demonstrate how to perform differential expression analysis on two groups of single cells. Lastly, we show how we can use the ...
C. Direct interaction between Escherichia coli RNA polymerase and the zinc ribbon domains of DNA topoisomerase I. J. Biol. Chem. 278, 30705–30710 (2003). Article CAS PubMed Google Scholar Merino, A., Madden, K. R., Lane, W. S., Champoux, J. J. & Reinberg, D. DNA topoisomerase...
N6-methyladenosine (m6A) is a post-transcriptional modification that controls gene expression by recruiting proteins to RNA sites. The modification also slows biochemical processes through mechanisms that are not understood. Using temperature-dependent (
However, both bulk RNA-seq and scRNA-seq still fail to preserve the spatial organization of cells and their associated transcripts within the tissue. Parallelly, the advent of Next Generation Sequencing (NGS) enabled parallel multiplexed analysis of DNA sequences on a massive scale—tens of thousan...