You can track CAR-T persistence real-time by labeling with luciferase or fluorescent proteins (e.g. GFP, etc.). What Sort of Models would be Suitable for CAR-NK Cell Therapies? Similar to CAR-T, models expressing the relevant target. Do You Use Fresh or Frozen CAR-Ts for a Large Scre...
The zwitterionic lipid cephalin enabled a sevenfold increase in luciferase expression as compared with the cationic lipid DOTAP. In addition, the effect of different administration routes needs to be assessed due to potential translational blockage caused by innate immune responses, as recently shown for...
When melanoma cells lose these differentiation antigens, they become more mesenchymal in appearance and highly invasive. Clinically, this also makes them harder to detect, as melanocytic antigens are used to detect melanoma metastases in lymph nodes and other organs. We have previously shown that ...
Xu et al. used an HEV3–Kernow replicon system in Huh7.5 cells (a RIG-I-defective hepatoma cell line) to show that the ectopic overexpression of RIG-I delivered by a lentivirus vector strongly reduced HEV3–Kernow replicon luciferase activity 48 h after transduction [53]. IRF1 and MDA5 ...