GL261 murine glioma cells are widely used as a syngeneic animal model of glioma, however, it has become common practice to transfect these cells with luciferase for fluorescent tumor tracking. The aim of this study was to compare the survival of mice injected with fluorescent or non-fluorescent...
However, median survival was significantly prolonged to 37 days in mice implanted with 5 × 104 GL261-Luc2 cells. Additionally, proteomic analyses revealed significantly elevated inflammatory cytokines in the supernatants of the GL261 Red-FLuc cells and GL261-Luc2 cells. Our data suggest that...
Objective:To construct cells stably expressing luciferase containing nonsense mutation for rapid screen of compounds which can induce readthrough of nonsense mutations. Methods:Plasmids pGL4-WT and p GL4-MUT were digested by endonuclease to give c DNA encoding wild type luciferase and nonsense mutant...
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描述: PUBCHEM_BIOASSAY: qHTS screen for small molecules that induce genotoxicity in human embryonic kidney (HEK293T) cells expressing luciferase-tagged ELG1. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID493106, AID493143] Format: BAO_0000019 Organism: Homo sapiens...
The NS1, NS3 proteins and dsRNA were detected in the replicon cells by immunofluorescence analysis. Furthermore, the anti-DENV inhibitors ribavirin and bromocriptine significantly reduced the luciferase activity in a dose-dependent manner. High-throughput screening with a compound library using the ...
T cellsMouse models for the study of pancreatic ductal adenocarcinoma (PDAC) are well-established and representative of many key features observed in human PDAC. To monitor tumor growth, cancer cells that are implanted in mice are often transfected with reporter genes, such as firefly luciferase (...
By facilitating the engraftment of human tumor and immune cells, HIS models provide a platform for studying tumor-associated immunology, immunotherapy drugs, and tumor-immune cell interactions. Its application extends to assessing immune-checkpoint inhibitors and cell therapies, in particular CAR-T cells...
This was improved by increasing the number of tumor cells injected. Tumor cells were found in femurs, spleen, kidneys, ovary, lungs, and uterus depending on the cell line. Mice survival was also highly affected by the tumor cell line engrafted. We observed that MOLM14 was the most ...
Imaging Luciferase-Expressing VirusesOptical imaging of luciferage gene expression has become a powerful tool to track cells and viruses in vivo in small animal models. Luciferase imaging has been used to study the location of infection...doi:10.1007/978-1-61779-340-0_6Barry, Michael A....