Methods Luciferase-expressing N. caninum (Nc1-Luc) was constructed using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9). After testing the luciferase expression and phenotype of the Nc1-Luc strains, the drug sensitivity of Nc...
for the GL261 and its luciferase-labeled cells. In addition, elevated TGF-β2 in GL261 luciferase-expressing cells is hypothesized to occur through a compensatory mechanism. It may resemble what was found by Ksendzovsky et al., whereby GL261 TGF-β1 secretion was found to be upregulated in...
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Objective:To construct cells stably expressing luciferase containing nonsense mutation for rapid screen of compounds which can induce readthrough of nonsense mutations. Methods:Plasmids pGL4-WT and p GL4-MUT were digested by endonuclease to give c DNA encoding wild type luciferase and nonsense mutant...
描述: PUBCHEM_BIOASSAY: qHTS screen for small molecules that induce genotoxicity in human embryonic kidney (HEK293T) cells expressing luciferase-tagged ELG1. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID493106, AID493143] Format: BAO_0000019 Organism: Homo sapiens...
coli cells expressing firefly luciferase was investigated. It was shown that bioluminescence intensity and time-course of the bioluminescent signal changed upon immobilization and depended on intracellular ATP concentration and permeability of the cell membrane....
Conditional bicistronic Cre reporter line expressing both firefly luciferase and beta-galactosidase. Mol Imaging Biol 2010.Ishikawa, T.O., and Herschman, H... TO Ishikawa,HR Herschman - 《Molecular Imaging & Biology》 被引量: 22发表: 2011年 A GFP-equipped bidirectional expression module well suit...
Construction of the PD-L1 Promoter-Luciferase Reporter Expressing Vector for Small Molecule Inhibitors ScreeningMajority of cancer cells up-regulate co-inhibitory molecule PD-L1 which inhibits T cell activation and proliferation, and allows cancers to escape from host immune surveillance, suggesting that ...
Taken together, our results demonstrate that this DENV subgenomic replicon cell system expressing a secretory luciferase gene can be useful for the high-throughput screening of anti-DENV compounds and the analysis of the replication mechanism of the DENV RNA....
Assay of ATP in intact cells of the facultative phototroph Rhodobacter capsulatus expressing recombinant firefly luciferase - Tomaso, Borghese, et al. - 2001Di Tomaso G, Borghese R, Zannoni D. Assay of ATP in intact cells of the facultative phototroph Rhodobacter capsulatus expressing recombinant ...