(B) Each TaqMan Advanced miRNA Assay employs a TaqMan minor groove binder (MGB) probe that specifically anneals to a complementary sequence between the forward and reverse primer sites. The MGB modification increases melting temperature without increasing probe length, a...
Quasi-quantitative assayLimit of blank (LoB)Multiparametric flow cytometry assays are long recognized as an essential diagnostic test for leukemias and lymphomas. Lacking Food and Drug Administration–approved standardized tests, these assays remain laboratory developed tests. The recently published guidelines...
(B) Each TaqMan Advanced miRNA Assay employs a TaqMan minor groove binder (MGB) probe that specifically anneals to a complementary sequence between the forward and reverse primer sites. The MGB modification increases melting temperature without increasing probe length, a...
PrimeTime™ qPCR Assay Selection Tool—use to select predesigned sequences for human, mouse, and rat targets. RealTime qPCR Design Tool—use to design primers, probes, and assays across exon boundaries for gene targets in species other than human, mouse, and rat. OligoAnalyzer Tool—use to...
Design Matters! Tips for Preparing Microscopy Samples Live Cell Imaging – An Overview for Beginners ibidi: The Versatile Toolbox for Cell-Based Assays Wound Healing and Migration Assays Angiogenesis and Tube Formation Assays Chemotaxis Assays Micropatterning as a Tool for Cell-Based Assay Ima...
Learn how to optimise your assay by evaluating and adjusting design elements to improve performance Assess the key performance parameters that impact the assay’s efficiency, technical dynamic range and reproducibility Receive recommendations for precise characterisation of assay sensitivity, specificity and ...
Another way to affect the enzyme is by lowering the pH of the solution. However, lactase is supposed to be able to work in the stomach. Would lowering the pH of the enzyme solution affect the enzyme? Design an Experiment to Test How pH Affects Enzyme Reactions. ...
An organization that develops and implements a solid NPI process positions itself closer to the success of a product. One obvious advantage is cost reduction; every decision in the product development process affects the cost be it brought by repeated revisions, validation testing, and design changes...
Design an Experiment to Test How pH Affects Enzyme Reactions. Briefly describe what effect you expect each of the following variables to have on enzymatic activity. 1. pH of 3.0 2. decrease in temperature 3. increase in substrate concentration 4. increase in enz ...
The format for this microplate-based assay typically utilizes a capture antibody specific for the desired protein, independent of the phosphorylation state. The target protein, either purified or as a component in a complex heterogeneous sample such as a cell lysate, is then bound to the antibody...