or comet assay (CA). DNA strand breaks (SB) were detected by the standard alkaline comet assay, while the modified version of the assay was performed by using the enzyme formamidopyrimidine DNA glycosylase (Fpg, kind gift from NorGenoTec AS, Norway) to specifically detect oxidized base lesions...
Check the amount of sample required per well (calculate volume to measure your samples in duplicates). Low sample volumes and precious samples are often a selection criterium. 5. SENSITIVTY – BIOMARKER CONCENTRATIONS TO BE EXPECTED Before choosing an assay, look into the validation data of the ...
These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to begin your project sooner by easily analyzing and troubleshooting experimental designs. PrimerQuest Tool—use to generate highly customized ...
A bi-directional Caco-2 permeability assay is performed where the transport of the compound is measured in the apical to basolateral direction as well as the
How to calculate t3/reverse t3 ratio – National Academy of Hypothyroidism This has been a quick discussion of a complex system. Not all aspects of thyroid testing and treatment have been addressed. Hopefully this discussion will help you understand why simply measuring TSH and T4 (a common prac...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
However, the dose-response curve may not extend between the controls (the NS controls are considerably lower than the Bottom plateau) as seen below. So, how do we calculate the IC50? The relative IC50 is estimated by considering the Bottom and Top plateaus obtained by implementing the 4PL mo...
Calculate the total cost after the change. Include the additional expenses directly attributed to the production or sale of the extra unit. Subtract the total cost before the change from the total cost after the change to find the incremental cost. ...
How to find a manufacturer in China How to choose a supplier in China How to calculate manufacturing overhead How to request a quotation from suppliers How to ensure product quality and lead time How to protect your intellectual property from your manufacturer And all the ins and outs of produ...
Explain how to calculate absorbance change per minute in ONPG enzyme activity. What is the model of enzyme activity that explains how a particular enzyme will only fit with one particular type of substrate? Why might a glucose test strip be used to determine the enzymatic...