Scientists and technicians often need to calculate the concentration of cells in a suspension. For example, when a patient gets his blood drawn at a doctor’s office, the laboratory can use certain methods to look for the amount of white blood cells in a given volume of blood. This gives ...
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If you know the concentration of enzyme sites you've added to the assay (Et) then you can calculate the catalytic constant Kcat. It is defined to equal Vmax/Et. Vmax and the Y values (enzyme velocities) are expressed in units of concentration per time, and Et must be entered in those ...
irrespective of who is doing the job. You will also need to calculate the amount of power you will need, and whether you will be employing people to do the work on your behalf. From what has been said above, it might not be possible to come up with a precise budget for your producti...
The difference in the amounts of standard MPs before and after pretreatment process was used to calculate the recovery of MPs during the analytical method. However, for such small MPs, it is difficult to spike a known mass of standard MPs that could be enumerated in the following steps. For...
I will then review the complications involved with measuring the free hormone levels and the efforts to calculate those levels based on estimates of binding constants and levels of both total hormone and total binding protein. In this review, the major focus will be on ...
For example, in an assay you may measure the fluorescence but would like to plot concentration units. Knowing before-hand the relationship between the amount of fluorescence generated and the concentration of target in a sample allows you to convert measured fluorescence response values to ...
CD47 recognized by its macrophage receptor SIRPα serves as a “don’t eat-me” signal protecting viable cells from phagocytosis. How this is abrogated by apoptosis-induced changes in the plasma membrane, concomitantly with exposure of phosphatidylserine
To calculate EC50 in the ISO dose-response assay, data were scaled to 100 for the entire dataset, and values obtained with and without GRK2 were fit to a sigmoidal curve (four-parameter logistic equation) with a variable slope. For the assay of the RH domain mutants, the transfected GRK2...
Skip to main contentSkip to article Journals & Books Help Search My accountSign in View PDF Download full issue Search ScienceDirect Marine Pollution Bulletin Volume 64, Issue 12, December 2012, Pages 2871-2876BaselineField studies using fish biomarkers – How many fish are enough?