as the standard sample, then the conclusion is that the test sample contains the same amount of protein as the standard sample. Because there was 10 µg of standard per well, one could report the determined concentration of tes...
Figure 4. Comparison of point-to-point and linear-fit standard curves.Interpolation and calculation for a test sample having absorbance 0.6 results in significantly different protein concentration values. In this case, the point-to-poin...
Values from IL6 lab I (outlier’s values) were excluded from the calculation. Empty Cell Empty CellVariance Components Empty Cell Empty CellPBMC-SP (24 h & 48 h)WB-AQ (24 h) TreatmentCytokineIntra-Lab (%)Inter-Lab (%)Intra-Lab (%)Inter-Lab (%) CD28-SAIFN- γ 29 71 35 65 IL...
A DNA Break Accumulation as an Assay for Enzymatic Incision The accumulation of DNA breaks due to incision at damage sites in the presence of DNA synthesis inhibitors is the basis of a rapid and convenient assay for repair activity. In various respects—simplicity, sensitivity, and freedom from ...
The validation target acceptance criteria should be chosen to minimize the risks inherent in making decisions from bioassay measurements and to be reasonable in terms of the capability of the art. When there is an existing product specification, acceptance criteria can be justified on the basis of ...
It also simplifies the calculation of survival curves and suppresses the influence of human errors in identification of all dead worms. Hands-free killing assay on a chip is compatible with the standard killing assay on a plate. As a proof of principle, we measured the survival of worms ...
Methods and Results:The present study reports an example where a shift in a BAP assay was detected by use of a patient pool and supported by a retrospective calculation of "patient mean", while the external QC and specific assay control material were unaffected by the shift. Conclusions:...
Accord- ingly, calculation of the relative matrix effects was unneces- sary. As shown in Table 2, the compound responses and the chromatographic behaviors (eg, column efficiency, retention times, and peak shapes) did not change significantly during and after 100 successive injections of the ...
Separating the head accurately from the rest of the comet is essential, since it strongly influences the calculation of the amount of DNA damage. Our first method for finding the comet head comes from the assumption that the head is the brightest region of the comet [19]. This method is ...
Fig. 4.β-glucosidase activity (at 22 °C) as a function of substrate concentration in grassland soils from Irvine, California, USA. Values are Mean ± standard error. SeeFig. 2for line calculation.Kmwas estimated at 32.77 μM substrate, andVmaxas 410 nmol min−1g−1. ...