Single-cell RNA sequencing (scRNAseq) is a powerful tool to describe cell types in multicellular organisms across the animal kingdom. In standard scRNAseq analysis pipelines, clusters of cells with similar transcriptional signatures are given cell type labels based on marker genes that infer ...
(2014). How deep is enough in single-cell RNA- seq? Nat. Biotechnol. 32, 1005-1006.Streets, A.M., and Huang, Y. (2014). How deep is enough in single-cell RNA-seq? Nat Biotechnol 32, 1005-1006.Streets AM, Huang Y. 2014. How deep is enough in single-cell RNA-seq? Nat. ...
Single-cell RNA-sequencing (scRNA-seq) technologies have allowed a breakthrough in this respect37. Although scRNA-seq can be performed in “bulk” with the use of small tubes and vials, this technology requires precise control of small volumes down to the picoliter scale. Requirements that ...
How does splicing of pre-mRNA occur? How are DNA, RNA, and proteins related in the cell? How is mRNA synthesized from DNA? Identify the three difference between DNA and RNA. Compare the tRNA and mRNA in terms of their functions and how they are synthesized within the cell. ...
RNA ReplicationRNA is replicated in the process of transcription of DNA, where mRNA (messenger RNA) is created through enzymatic catalysis of a polymerase complex reading the coding strand of a parent DNA sequence.Answer and Explanation: RNA, in modern organisms, does not necessarily self-replicate...
cell death (Sylvain-Bonfanti et al.2024). So, as in the human anaesthesiology, concentration, type of application and time duration matter. Application of diethyl ether induced strong reprogramming of gene expression inA. thaliana. The RNA-seq analysis showed that more than half out of 21,500 ...
(FC) threshold between 2 and 4 was necessary to obtain replicable DE genes. We wondered if a similar threshold would hold for single-cell RNAseq and how aggregation across datasets would improve the threshold for FC and the area under the receiver-operator curve (AUROC), a statistic routinely...
Transcriptomics by single-cell or single-nucleus RNA-sequencing (scRNA-seq or snRNA-seq) is now the most widely used approach to generate cell type taxonomies or atlases from many species, tissue organs, and brain regions, due to its comprehensiveness and high dimensionality (i.e., profiling ...
Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions. Cell Stem Cell 20, 858–873 (2017). Article ADS CAS PubMed Google Scholar Jorgensen, A. et al. Nodal signaling regulates germ cell development and establishment of seminiferous cords in the ...
et al. Single-cell RNA-seq reveals the transcriptional landscape and heterogeneity of aortic macrophages in murine atherosclerosis. Circ. Res. 122, 1661–1674 (2018). Article CAS PubMed Google Scholar Winkels, H. et al. Atlas of the immune cell repertoire in mouse atherosclerosis defined by ...