How does Coomassie staining identify the protein band? Briefly explain. Describe an experiment that could test the hypothesis that an enzyme binds to a substrate when it reacts to a lab involving food and enzymes. A. Explain why a glucose test strip ma...
Step 2: Transfer the resin from step 1 to a reaction column and swell it in DCM. Use 20% PIP/DMF to remove Fmoc. Wash with DMF and DCM alternately. Test with ninhydrin, and the result should be positive. Dissolve Fmoc-Trp(Boc)-OH and HOBT in DMF/DCM=3/1 as the reaction solvent....