In the case of porous surfaces, fingerprints can be detected using a solution called ninhydrin. This solution reacts with amino acids present in sweat and turns purple due to the formation of Ruhemman’s purple. Sometimes CSIs also cyanoacrylate or super glue...
How does Coomassie staining identify the protein band? Briefly explain. Staining: The technique used to improve the visualization of the sample by increasing the contrast of the image is known as staining. The process involves the coloring of the sample or its background. This technique can be...
Answer and Explanation:1 SDS-PAGE is a form of gel electrophoresis. The SDS is a detergent that will cause a denaturing (unfolding) of the protein, and then gel... Learn more about this topic: Common Laboratory Equipment: Types & Uses ...
Step 2: Transfer the resin from step 1 to a reaction column and swell it in DCM. Use 20% PIP/DMF to remove Fmoc. Wash with DMF and DCM alternately. Test with ninhydrin, and the result should be positive. Dissolve Fmoc-Trp(Boc)-OH and HOBT in DMF/DCM=3/1 as the reaction solvent....