How does Coomassie staining identify the protein band? Briefly explain. Staining: The technique used to improve the visualization of the sample by increasing the contrast of the image is known as staining. The
The amino acid analysis data indicates that proline is not easily detected by reaction with ninhydrin. Suggest a reason. Proline's structure has a unique feature not found in other amino acids. What is it? How and why are proteins added to sugars? Which forces and...
Step 2: Transfer the resin from step 1 to a reaction column and swell it in DCM. Use 20% PIP/DMF to remove Fmoc. Wash with DMF and DCM alternately. Test with ninhydrin, and the result should be positive. Dissolve Fmoc-Trp(Boc)-OH and HOBT in DMF/DCM=3/1 as the reaction solvent....
Answer and Explanation:1 SDS-PAGE is a form of gel electrophoresis. The SDS is a detergent that will cause a denaturing (unfolding) of the protein, and then gel... Learn more about this topic: Common Laboratory Equipment: Types & Uses ...