MacFadyen, DA (1950) On the mechanism of the reaction of ninhydrin with α-amino acids. J Biol Chem 186: pp. 1-12Mac FD. On the mechanism of the reaction of ninhydrin with alpha-amino acids. I. Absorption spectr
Its reaction formula is: Mechanism When a free amino acid reacts chemically with ninhydrin, which acts as an oxidizing agent, its amino group undergoes oxidative deamination, leading to the production of CO2, NH3, an aldehyde, and a reduced form of ninhydrin known as hydrindantin. Subsequently...
Ninhydrin (2,2-dihydroxyindane-1,3-dione) reacts with primary and secondary amino compounds to form characteristic-colored compounds. Since its discovery by Ruhemann in 1910 [1], this colorimetric reaction has been widely used for the detection of amino acids, peptides, proteins, and amines. ...
In the same year, Ruhemann observed ninhydrin's reaction with amino acids. In 1954, Swedish investigators Oden and von Hofsten proposed that ninhydrin could be used to develop latent fingerprints. Uses Ninhydrin is an amino acid-sensitive reagent for the development of latent fingermarks on ...
Ninhydrin reacts with primary and secondary amines (including amino acids, proteins, and peptides) to give a dark purple product known as Ruhemann's purple (RP) . As the eccrine component of a latent mark deposit contains amino acids, this reaction can be exploited as a means of developing...
Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines. Reaction with sarcosine or proline gives azomethine ylides. Application: It can be used for the detection of peptides, amino acids, ...
The functional groups analyzed and detected by Ninhydrin stain are the Amine groups. The amine groups associated with the alpha-amino acids are...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your tough homework...
The ninhydrine reaction can also be applied to the quantitative determination of certain other amino-acids. Thus one of us (Laine) has developed the method for the determination of leucine by means of isovaleraldehyde formed in the reaction. We are continuing our work for the determination of ...
NEGATIVE RESULTS WITH THE NINHYDRIN REACTION AS A TEST FOR AMINOACIDS IN THE SERUM OF NEPHRITICS AND OTHERSThe use by Abderhalden of "ninhydrin" (triketohydrindenhydrate) as an indicator in his test for pregnancy, and especially his experimental work with Lampe,1 in which ninhydrin was used...
F. 1953 The effect of relative humidity on the reaction of ninhydrin with amino acids on paper chromatograms. Can. J. Chem., 31, 484-487.Wellington , E. F. : The Effect of Relative Humidity on the Reaction of Ninhydrin with Amino Acids on Paper Chromatograms. Canad. J. Chem. 31 ,...