Its reaction formula is: Mechanism When a free amino acid reacts chemically with ninhydrin, which acts as an oxidizing agent, its amino group undergoes oxidative deamination, leading to the production of CO2, NH3, an aldehyde, and a reduced form of ninhydrin known as hydrindantin. Subsequently...
The meaning of NINHYDRIN REACTION is a reaction of ninhydrin with amino acids or related amino compounds used for the colorimetric determination of amino acids, peptides, or proteins by measuring the intensity of the blue to violet to red color formed or
H. S. The ninhydrin reaction with amino acids and ammonium salts. J. Biol. Chem. 1916, 25, 319-335. (18) Kim, Y. J.; Uyama, H. Tyrosinase inhibitors from natural and synthetic sources: Structure, inhibition mechanism and perspective for the future. Cell. Mol. Life Sci. 2005, 62, ...
In the same year, Ruhemann observed ninhydrin's reaction with amino acids. In 1954, Swedish investigators Oden and von Hofsten proposed that ninhydrin could be used to develop latent fingerprints. Uses Ninhydrin is an amino acid-sensitive reagent for the development of latent fingermarks on ...
The reaction of ninhydrin with primary amino groups to form the purple dye now called Ruhemann's purple (RP) was discovered by Siegfried Ruhemann in 1910. In addition, imines such as pipecolic acid and proline, the guanidino group of arginine, the amide groups of asparagine, the indole ring...
The mechanism of the reaction for quantitative determination of amino acids with ninhydrin has been extended to explain the dependence of color formation on hydrindantin concentration. Voltammetric techniques have been used to study the ... PJ Lamothe,McCormick, Patrick G. - 《Analytical Chemistry》...
This reaction is unique among chromogenic reactions in that at pH 5.5 it results in the formation of the same soluble chromophore by all primary amines which react, be they amines, amino acids, peptides, proteins, and even ammonia. Because the chromophore is not chemically bound to the ...
: a reaction of ninhydrin with amino acids or related amino compounds used for thecolorimetric determination of aminoacids, peptides, or proteins by measuring the intensity of the blue to violet to red color formed or for the quantitative determination of amino acids by measuring the amount of ...
THE micro-determination of 伪-amino-acids with ninhydrin in n -butanol is highly sensitive, but often inaccurate due to the retardation of colour formation and the production of various red or blue colours instead of the purple compound usually obtained. It was found that traces of cations had...
Condensation of ninhydrin with aldehydes and primary amines to yield highly fluorescent ternary products: I. Studies on the Mechanism of the Reaction and S... It was found that ninhydrin, certain aldehydes, and primary amines condense to yield highly fluorescent products. 伪-Amino acids and peptide...