PURPOSE:To obtain a ninhydrin reagent for quantitative analysis of amino acid having no danger generating a noxious substance inducing blood disorder or teratogenesis as a byproduct, by using specific 1-alkoxy-2-alkanol alone or in combination with dimethylsulfoxide. CONSTITUTION:1-Alkoxy-2-alkanol ...
For complete reaction, the beads remain yellow; for incomplete reaction, the beads turn blue. For nearly complete reaction, the beads will be very light blue. Even 5 µmol/g of free terminal amine group can still be detected by this method. View chapter Chapter CLINICAL ANALYSIS | ...
The present report describes the development of and results with a modified ninhydrin colorimetric method for the determination of free plasma -amino nitrogen. Precipitation of the plasma proteins with a hydrochloric acid-sodium tungstate reagent, filtration of the deproteinized filtrate through glass woo...
A New Method for the Detection of Peptides and Similar Compounds on Paper Chromatograms SINCE the first introduction 1 of paper chromatography for the separation and identification of amino-acids and peptides, ninhydrin has been used almost e... HN Rydon,PWG Smith - 《Nature》 被引量: 166发...
The objectives of this study were to determine the feasibility of measuring amino acid exosmosis by ninhydrin as a test for high temperature injury to wheat and evaluate the suitability of the method for use on maturing plants. EVALUATION OF A NINHYDRIN PROCEDURE FOR MEASURING MEMBRANE THERMOSTABILIT...
What is protein A? Describe one method of detecting it. Select the correct answer. When you perform a negative control, you use a chemical that you know should give you a [{Blank}] result in the test. The negative control for the ...
Introduction: A simple and reliable high performance liquid chromatographic method has been developed for the quantitative determination of pregabalin in bulk and dosage form. Pregabalin, a gamma amino butyric acid analogue, has negligible sensitivity to UV or fluorescence detection. Hence, it has been...
A Shortened Method for the Fluorometric Determination of Phenylalanine As a result of a study of the classic ninhydrin reaction with amino acids (which results in the production of Ruhemann's purple), and the fluorescence reac... Ambrose John A - 《Clinical Chemistry》 被引量: 26发表: 1969年...
asthereagentuniversallyusedforthedetectionofprints onporoussurfacessuchaspaper.Itprovidesameans ofdetecting,withhighsensitivitytheaminoacidand ammoniacomponentsoffingerprintsandisrelatively cleanandsimpletoapply.Thechemicalreactions involvedhoweverarecomplexanditseffectiveness ...
This article describes an improved method for automated amino acids analysis based on the use of sodium borohydride as reducing agent. The reagent is highly stable and forms no precipitate in the flow-through system of the analyser.doi:10.1002/(sici)1521-3803(19991201)43:6<410::aid-food410>...