Results conclusively indicated that 2 moles of ninhydrin quantitatively react with 1 mole of amino acid, implying that a given weight of protein requires a certain minimum concentration of ninhydrin in order to initiate the reaction and form Ruhmann's purple. Optimum Ruhmann's purple values for ...
A convenient microtitration method for the estimation of amino acids. cysteinecystineestimationhydroxyprolinemethodologytechniquesThe amino-acids were allowed to react with ninhydrin at pH 2.5 in a micro-aeration tube. The excess... AE Sobel,A Hirschman,L Besman - 《Journal of Biological Chemistry》...
A simple method is proposed for the determination of the ( alpha plus epsilon ) amino groups of wool by reaction with ninhydrin. The method gives good repr... J Knott,M Grandmaire,J Thelen 被引量: 11发表: 1981年 Influence of starter culture on total free aminoacids concentration during ri...
The most sensitive and accurate method in our hands is a colorimetric ninhydrin method based on that of Moore and Stein7. Applying the method directly to pieces of filter paper, there is a high and variable `amino-acid' value, due mainly to the presence of absorbed ammonia. The colour ...
On the mechanism of the reaction of ninhydrin with alpha-amino acids. I. Absorption spectra of ninhydrin and certain derivatives Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both amm... DA Macfa...
The fractions thus obtained were then examined after hydrolysis by comparing their amino-acid 'spectra'. These were obtained by using a method' which has been applied here to the characterization of proteins. Ninhydrin-developed paper chromato- gram strips were passed through a photo-electric ...
A new, accurate, precise and economical spectrophotometric method has been developed and validated for the determination of Amlodipine Besylate in bulk drugs. This method is based on the reaction of ninhydrin with primary amine present in the Amlodipine Besylate in the presence of Sodium Bicarbonate....
The proposed procedure compares favorably with a corresponding ninhydrin technic for plasma amino nitrogen. The mean plasma amino nitrogen values and range for a group of adults (age 18-50 years) by the proposed method is 5.56 mg./100 ml. (3.6-7.0 mg./100 ml.) compared to 4.12 mg./100...
phenolic compounds, flavonoids, saponins, carbohydrates, protein and amino acids, fixed oils and fat test. The total phenolic content ranged from 280.5 to 287.5 mg/g of dry weight of extract, expressed as Gallic acid equivalents. The total flavonoid concentrations varied from 264.4 to 267.3 mg/g...
AMINO ACIDSINDICATORS AND REAGENTSIT is well known that the development of a purple colour (Ruhemann's purple) on reaction of ninhydrin with primary amino (NH2) groups of proteins occurs even when the protein is not in solution. Thus, Pearse1 describes attempts to use the ninhydrin reaction ...