For example, rithium acetate 264g (4mol) is dissolved in water 600ml, and next, acetic acid 200ml (3.5mol) is added, and further, water is added to make the whole of the quantity of 1l, thus obtaining buffer liquid. Meanwhile, ninhydrin 20g and sodium borohydride 75mg are dissolved...
Ninhydrin suitable for amino acid detection; CAS Number: 485-47-2; EC Number: 207-618-1; Synonyms: 1,2,3-Indantrione monohydrate,2,2-Dihydroxy-1,3-indanedione,Trioxohydrindene monohydrate at Sigma-Aldrich
Rapid comprehensive amino acid analysis by liquid chromatography/tandem mass spectrometry: comparison to cation exchange with post-column ninhydrin detection Ion-exchange chromatography with ninhydrin detection remains the gold standard for detecting inborn errors of amino acid catabolism and transport. Disadva...
Usingamino acidanalysis (AAA), the relative amount (ratio) and absolute content ofamino acidsin a peptide can be determined. However, the use of proton NMR was proposed as an identity test for peptides. This chapter evaluates the usage of proton NMR as an alternative toAAAas an identity te...
B. Apparatus (k) Amino acid standard kit .—To calibrate amino acid analyzer; (a) Amino acid analyzer.—Ion-exchange resin with ninhydrin available from Aldrich Chemical Co., Inc., 1001 West Saint Paul post-column derivatization. Ave, Milwaukee, WI 53233, USA. (b) Analytical balance .—...
take lml of this product, add 10ml of water, shake well, add about 3mg of ninhydrin, heat, the solution shows blue purple. in the chromatogram recorded under the content determination item, the retention time of each amino acid peak in the test solution should be consistent with the reten...
Amino acid abnormalities are observed in a broad spectrum of inherited metabolic diseases, such as disorders of amino acid metabolism and transport, organic acidemias, and ureagenesis defects. Comprehensive analysis of physiologic amino acids in blood, u
This study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [c
Concentrations were measured after reaction with ninhydrin at 570 nm, except for proline, which was measured at a wavelength of 440 nm. The same high-performance liquid chroma- tography column was used for all samples from an individual animal. Reproducibility within the same column had a ...
-octahydro-1H-indole-2(S)-carboxylic acid (250 mg) as a white powder. TLC, Rf=0.33 (Developing Solvent: B, Detection: ninhydrine). EXAMPLE XIV1-[N-[1(S)-carboxy-5-(4-piperidyl)pentyl]-L-alanyl]-(2α,3aβ,7a.b eta.)-octahydro-1H-indole-2(S)-carboxylic acid ...