Ninhydrin is a chemical that reacts with amino acids in proteins to produce a purple color. The ninhydrin test was performed on three substances: distilled water, apple cider, and egg whites (which contain albumin protein.) Identify each o...
Explain the controls for the biuret and ninhydrin tests. What is the purpose of each? What are the expected results of each control? What is the principle behind the LDH enzyme assay in the presence of its substrates or cofactors? What is the consequence of lack of r...
Step 2: Transfer the resin from step 1 to a reaction column and swell it in DCM. Use 20% PIP/DMF to remove Fmoc. Wash with DMF and DCM alternately. Test with ninhydrin, and the result should be positive. Dissolve Fmoc-Trp(Boc)-OH and HOBT in DMF/DCM=3/1 as the reaction solvent....
including the atoms, molecules, and ions that make up compounds and their composition, structure, behaviour, and changes that occur when they interact with other things.
calibrator curve in aqueous solution was determined using the ninhydrin reaction (specific for amine groups); in the CE-LIF method, homocystine was diluted in carbonate buffer (pH 9.5), and the calibrator curve in plasma solution was determined using IAF, which reacts specifically with thiol ...
The amino acid analysis data indicates that proline is not easily detected by reaction with ninhydrin. Suggest a reason. What are the main structural features of an amino acid? Why can a glucose test strip be used to determine the enzymatic activity of lacta...
Explain the controls for the biuret and ninhydrin tests. What is the purpose of each? What are the expected results of each control? How would you expect the results of starch hydrolysis to change if you will add glucose to the medium along with ...