Functional expression was tested by immunizing the A2Kb mice with an HLA-A*0201 restricted hepatitis C peptide (VLSDFKTWL) and corresponding pentamer staining for peptide specific CD8 T cells. There was significantly increased pentamer(+) CD8+ T cells in the immunized A2Kb mice indicating ...
The results demonstrated that peptides of ZnT8 containing residues 107-115,115-123 and 145-153 could elicit specific CTLs in vitro, and induce diabetes in mice. The results suggest that these specific peptides are novel HLA-A*0201-restricted CTL epitopes, and could have therapeutic potential ...
Simultaneous immunization of both CMV proteins, using DNA vaccine priming followed by rAAV boost, induced antibody (Ab) response, CD8 lymphocytes with cytotoxic function, and detectible binding of the cognate peptide epitopes for human HLA A*0201 restriction using tetramer technology....
HLA-A*0201-BSP融合基因 1. The present study was aimed to construct the expression vector of recombinant HLA-A*0201-BSP fusion gene for preparing HLA-A2 tetramers. 本研究构建重组HLA-A*0201-BSP融合基因的表达载体,以制备HLA-A2四聚体。
2) HLA-A*0201 transgenic mice HLA-A*0201转基因小鼠 3) HLA-A*0201-BSP fusion gene HLA-A*0201-BSP融合基因 1. The present study was aimed to construct the expression vector of recombinant HLA-A*0201-BSP fusion gene for preparing HLA-A2 tetramers. ...
本发明属于生物技术开发与应用研究领域,公开了HLA-A0201限制性抗MUC-1抗原特异性CTL的制备方法.该方法通过单采收集外周单个核细胞,富集纯化CD8+T淋巴细胞;用负载HLA-A0201限制性MUC-1抗原多肽的成熟树突状细胞刺激CD8+T淋巴细胞,用rhIL-2和rhIL-7促进T细胞生长;用Tetramer标记法和流式细胞分选术纯化目标CTL;用固相...
FIGURE 1. Immunogenicity of the HLA-A*0201-binding MOG pep- tides. Immunogenicity of the high-affinity (f), intermediate-affinity (u), and low-affinity (Ⅺ) HLA-A*0201-binding MOG peptides. Groups of HLA-A*0201 mice were immunized with a given HLA-A*0201- binding MOG peptide and the...
HLA-A11, HLA-A24 and HLA-b40 transgenic mice. Of note, HLA-A2 restricted, HPV18 E7 peptide (aa7-15)- and HPV18 E6 peptide (aa97-105)-specific epitopes are endogenously processed by HPV18 positive Hela-AAD (HLA-A*0201/Dd) cells. Finally, we found that injection of DNA plasmids ...
HLAA*0201/Kb及MAGE3基因共 转 【摘要】目的:为了用HLAA2.1转基因鼠制备荷瘤模型以评价 肿瘤疫苗的免疫效果,制备共表达HLAA*0201/Kb嵌合基因和 MAGE3的B16黑色素瘤细胞。方法:采用基因共转染的方法, 将HLAA*0201/Kb和MAGE3转染到B16黑色素瘤细胞
to predict potential HLA-A*0201-restricted CD8+T-cell epitopes. Candidate CD8+T-cell epitopes and their variant peptides in D2V, D3V, D4V were tested for their affinity to the HLA-A*0201 molecule, and for their capacity to induce CD8+T-cell responses in HLA-A*0201 transgenic mice. ...