Induction of melanoma-reactive CTL in HLA-A*0201/Kb transgenic mice by vaccination with peptides derived from MAGE2 and MART1Compelling new evidence supports the idea that regulatory T cells play a major role in our immune system. Several subsets of these regulators have been identified recently...
HLA-A*0201-BSP融合基因 1. The present study was aimed to construct the expression vector of recombinant HLA-A*0201-BSP fusion gene for preparing HLA-A2 tetramers. 本研究构建重组HLA-A*0201-BSP融合基因的表达载体,以制备HLA-A2四聚体。
Some class I MHC genes encode nonclassical (ie, not CD8-binding) MHC molecules, such as HLA-G (which may play a role in protecting the fetus from the maternal immune response) and HLA-E (which presents peptides to certain receptors on natural killer [NK] cells).I 类 MHC 分子以跨膜糖...
HLAA*0201/Kb及MAGE3基因共 转 【摘要】目的:为了用HLAA2.1转基因鼠制备荷瘤模型以评价 肿瘤疫苗的免疫效果,制备共表达HLAA*0201/Kb嵌合基因和 MAGE3的B16黑色素瘤细胞。方法:采用基因共转染的方法, 将HLAA*0201/Kb和MAGE3转染到B16黑色素瘤细胞
目的:应用T2细胞结合实验以及亲和力稳定性实验鉴定由网络表位预测服务和通用原则预测得到的乙肝病毒X蛋白(HBx)来源的HLA-A0201限制性CTL表位.方法:选择中国人群最常见的B,C基因型中的adw,adr血清型的乙肝病毒X基因序列,通过网络在线表位筛选服务,分析得到共同并且得分较高的表位,并根据超基序,延展基序及量化基序方案等...
to predict potential HLA-A*0201-restricted CD8+T-cell epitopes. Candidate CD8+T-cell epitopes and their variant peptides in D2V, D3V, D4V were tested for their affinity to the HLA-A*0201 molecule, and for their capacity to induce CD8+T-cell responses in HLA-A*0201 transgenic mice. ...
Four weeks after the second immunization, mice were infected with PR8 virus. NP-specific CD8 T cells recruited in the lungs after the infection were characterized by flow cytometry. (a) Numbers of NP-specific CD8+ T cells determined using Pro5® MHC Pentamers. Data are from individual ...
Sequence alignment of BAC RP11-10A19 revealed that this BAC clone carries TAP1*0101 and TAP2*0201, both of which occur at high frequencies in different human populations31,32. By using PCR screening, two founder mice (F2 and F14) carrying all six human genes were established (Fig. 1...
Recognition of human proinsulin leader sequence by class I-restricted T-cells in HLA-A*0201 transgenic mice and in human type 1 diabetes. OBJECTIVE: A restricted region of proinsulin located in the B chain and adjacent region of C-peptide has been shown to contain numerous candidate epitopes ....
Using mouse models, it has been confirmed that one of the homologous genes for HLA I molecules in mice, H-2D, shows specific selectivity for the secondary structure of its binding peptide, suggesting the specific role of the peptide secondary structure in MHC I molecule binding affinities62. ...