Results The paired plasma specimens collected from 219 study participants (mean age 37.6 years, SD = 9.4) were tested by Abbott m2000 Real Time PCR and GeneXpert HIV-1 Quant assays. Concordance between Abbott and HIV-1 quant assays The percentage of concordant samples in both the assays is ...
Two assays based on real-time PCR are avail... JC Karasi,F Dziezuk,L Quennery,... - 《Journal of Clinical Virology the Official Publication of the Pan American Society for Clinical Virology》 被引量: 32发表: 2011年 Laboratory evaluation of the Liat HIV Quant (IQuum) whole-blood and ...
For participant OM5346, HIV-1 RNA was extracted from QVOA outgrowth viruses and pre-ART plasma, after which gp160 (QVOA) and gp120/Pol (plasma) were amplified from endpoint-diluted templates by nested RT-PCR using HIV-1-specific primers and high fidelity enzymes. For participants with su...
Plasma HIV-1 RNA levels were measured at BIDMC using the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, version 2.0 (lower limit of quantification (LLoQ) = 23 RNA copies per ml), until it was replaced with the Hologic Aptima HIV-1 Quant Assay (LLoQ = 32 RNA copies per ml)...
RNA was extracted from 1.0 ml of plasma using the Qiagen QIAsymphony SP automated extractor followed by amplification and detection of HIV-1 LTR on the Qiagen Rotor-Gene Q real-time PCR instrument (Qiagen GmbH, Hilden, Germany). The LLOQ is 45 copies/ml and the upper range of quantitation...
Plasma viral RNA levels were measured by quantitative real time RT-PCR for SIV Gag (Duke Human Vaccine Institute) with a lower limit of detection of 500 RNA copies/mL. 4.3. Cloning of SHIV_KB9-B3 To generate a molecularly cloned transmitted founder envelope SHIV, we used the HIV-SIV archi...
We assessed the correlation between HIV-RNA viral load in blood (BPVL) and seminal plasma (SPVL) in a cross-sectional cohort of 119 asymptomatic, antiretroviral-naive and experienced HIV-1 subjects (BPVL < 50 copies/mL for minimum 12 months and stable on one drug regimen). The cohort ...
RNA purity and quantitation were done on the BioTek's Synergy Neo2 multimode plate reader using the Take3 adapter. Real-time PCR was done using the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher cat# 4444432). Optimized primer–probe TaqMan assays were obtained from Thermo Fisher for ...
Plasma HIV-1 viral load was measured at BIDMC by real-time PCR assay using the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, v.2.0 (LLoQ = 23 copies ml–1), until it was replaced with a transcription-mediated amplification assay using the Hologic Aptima HIV-1 Quant Assay (LLoQ...
to treatment-monitoring is required in LMICs if the UNAIDS “third 95” targets are to be achieved by 2030. Here, we review the current challenges of viral load testing and present the case for greater utilization of Laboratory-based assays that quantify intracellular HIV-1 RNA and/or DNA to...