反应(RT—PCR)半定量早期捡测HIv一1nefRNA.现报道 如下. 材料和方法(1)主要材料和试剂:RNA提取试剂贿自美 国TEL—TEST公司.RNA纯化试剂:酚,氯仿为美国Sigma 公司产品.无RNA酶的DNA酶I及淋巴细胞分层液贿自美 国Gibco公司.RT—PCR试剂盘购自美国PE公司.基因弓I ...
Comparative performance of the new Aptima HIV-1 Quant Dx assay with three commercial PCR-based HIV-1 RNA quantita- tion assays. J Clin Virol 2015; 69:56-62.Hopkins M, Hau S, Tiernan C, et al.Comparative performance of the new Aptima HIV-1 Quant Dx assay with three commercial PCRbased...
Quantitative measurement of HIV-1 RNA levels in plasma (‘viral load’) plays a central role in clinical management. The choice of assay platform can influence results and treatment decisions. Objective To compare the analytical performance of the new TMA-based Hologic Aptima® HIV-1 Quant Dx ...
Plasma HIV-1 RNA levels were measured at BIDMC using the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, version 2.0 (lower limit of quantification (LLoQ) = 23 RNA copies per ml), until it was replaced with the Hologic Aptima HIV-1 Quant Assay (LLoQ = 32 RNA copies per ml)...
Viral RNA was eluted in 400 µL and divided into aliquots for further use. Viral load determinations were carried out with the AptimaTM HIV-1 Quant Dx Assay and the Panther System for molecular diagnostics (Hologic®, Marlborough, MA). A PCR with primers PCR1 and PCR2 (Supplementary ...
Results The paired plasma specimens collected from 219 study participants (mean age 37.6 years, SD = 9.4) were tested by Abbott m2000 Real Time PCR and GeneXpert HIV-1 Quant assays. Concordance between Abbott and HIV-1 quant assays The percentage of concordant samples in both the assays is ...
The results are shown in Figure 1. The coefficient of determination of CD4 cell decline rate on presenting plasma HIV RNA measurement was 0.06 (95% CI, 0.04-0.08), closely resembling the findings in our primary data set. The low predictive value of presenting plasma HIV RNA level on ...
Two clusters passed the BIC test and were used as constraints on the folding using RNAstructure. Structural models are colour-coded by normalized DMS reactivity; bases not covered by the region of PCR are coloured in grey. Data used to construct models are representative data from n = 2 ...
To test the potential role of Pom121 in HIV-1 replication, Pom121 was downregulated in 293T cells with RNA interference. The 293T cells were transfected with siRNA targeting Pom121 and Pom121 protein shown at the size of 145 kDa was reduced 72 h posttransfection (Fig. 1A). Then, ...
There has been a surge in the emergence of HIV-1 drug resistance in Low and Middle-Income Countries (LMICs) due to poor drug-adherence and limited access to viral load testing, the current standard for treatment-monitoring. It is estimated that only 75%