This chapter describes the quantitation of human immunodeficiency virus-1 (HIV-1) RNA in plasma or serum samples. The polymerase chain reaction (PCR) has been used to quantitate levels of RNA and DNA from a variety of samples. A variation in the use of an internal control is the ...
To monitor viral RNA and MS cDNA, quantitative PCR assay was achieved with 125 ng of tRNA-equivalent virions or 125 ng of cellular DNA samples extracted from cells transfected with either wt or mutant pNL4-3 plasmids, or with empty plasmid as controls (mock). The qPCR was achieved ...
The quantitative viral outgrowth assay (QVOA) provides a precise minimal estimate of the reservoir of resting CD4(+) T-cell infection (resting cell infection [RCI]). However, the variability of RCI over time during antiretroviral therapy (ART), relevant to assess potential effects of latency-rev...
Plasma HIV-1 RNA levels (red line; right y-axis) and bNAb serum concentrations (VRC07-523LS (green), PGDM1400 (blue) and PGT121 (red); left y-axis) of group 2 participants. Gray-shaded areas indicate time on ART. Participant 65021 resumed ART but was lost to follow-up (LTFU),...
Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay J Acquir Immune Defic Syndr Hum Retrovirol (1995) B. Van Gemen et al. The one-tube quantitative HIV-1 RNA NASBA: precision, accuracy and application PCR Meth Appl (1995) D.G. Murphy...
ASSESSMENT OF CYTOKINE VALUES IN SERUM BY RT-PCR IN HIV-1 INFECTED INDIVIDUALS WITH AND WITHOUT HIGHLY ACTIVE ANTI-RETROVIRAL THERAPY (HAART). J. Venom. Anim. Toxins incl. Trop. Dis., 2008, 14, 4, p. 690 instructions. DNase digestion of RNA solution was carried with DNase (RQ1 RNase-...
Human Serum (HS), Fetal Bovine Serum (FBS) and Cytokine Treatment Harvest and Staining for Flow Cytometry RNA Isolation cDNA Synthesis RNA Measurement by Real-Time Quantitative Polymerase Chain Reaction (PCR) Results Gating Strategies for Flow Cytomet...
After the second M10 cell infusion, a rapid decline in CA HIV-1 RNA occurred again, with the median value decreasing from 522 on Day 30 to 311 on Day 40 (P = 0.0071). When these results were correlated with the plasma viral loads, we found that successful latency reversal might ...
We quantified late-RT, 2-LTR and two-step Alu PCR in control or RPLP1 overexpressing MT-4 cells infected with HIV-1, which represent the HIV-1 reverse transcription, nuclear entry and integration, respectively31,32. The results showed that RPLP1 over-expression had no effect on proviral ...
2.5.3. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction The ventricle and atrium regions of the mouse heart were isolated from each subject and homogenized in TRizol reagent according to manufacture protocol (Thermo Fisher Scientific) followed by Qiagen Clean up kit (Qiagen, Germanto...