To address whether HIC1 protein is recruited to the specific motifs in miR-23a ~ 27a ~ 24-2 and miR- -23b ~ 27b ~ 24-1 promoters, we performed chromatin immu- noprecipitation (ChIP) assays in MCF-7 cells. As anticipated, ChIP assays using antibody against HIC1 showed robust PCR ...
In the normal and neoplastic tissue from Apc þ /D716 mice, however, this may be due to a chromatin-based repressive mechanism surrounding the Hic1a region, as there was no increase in DNA methylation for this genotype (Figure 4). Apc þ /D716 polyps and tumors from DH mice showed ...
Silenced Tumor Suppressor Genes Reactivated by DNA Demethylation Do Not Return to a Fully Euchromatic Chromatin State Histone H3 lysine 9 (H3K9) and lysine 27 (H3K27) trimethylation are properties of stably silenced heterochromatin whereas H3K9 dimethylation (H3K9me2) is i... KM Mcgarvey,JA Fahr...
Reporter assay and chromatin immunoprecipitation (ChIP) assay confirmed that HIC1 bound to the proximal CIITA type III promoter (鈭 545/鈭 113); mutation of a conserved HIC1 site within this region abrogated CIITA trans-repression. More important, depletion of HIC1 with small interfering RNA ...
The Hi-C matrix has to be corrected to remove GC, open chromatin biases and, most importantly, to normalize the number of restriction sites per bin. Because a fraction of bins from repetitive regions contain few contacts it is necessary to filter those regions first. Also, in mammalian genome...
Quantitative RT-PCR, bisulfite pyrosequencing, colony formation assay, chromatin immunoprecipitation, and RNA interference was used. RESULTS: HIC1 was generally underexpressed regardless of the hyperparathyroid disease state including multiple parathyroid tumors from the same patient, and overe...
Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control. Oncogene 2009, 28:651-61.Zhang B, Chambers KJ, Leprince D, Faller DV, Wang S: Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1- mediated ...
Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control. Oncogene 28:651-661.Zhang B et al (2009) Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control. ...
Hic1 protein has been shown to interact directly with Atoh1 by chromatin immunoprecipitation, and its expression results in a reduction of Atoh1 expression. We conclude that Hic1 gene silencing genetically interacts with Ptch1 mutation to promote the formation of MB. Given that Atoh1 is also a...
KJLeprinceDFallerDVEBSCO_AspOncogeneZhang,B., Chambers,K.J., Leprince,D., Faller,D.V. and Wang,S. (2009) Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control. Oncogene, 28, 651-661....