(d) Immunohistochemistry using an antibody against Sox9 for paraffin-embedded polyps from small intestine (SI) and large intestine (LI). Genotypes of mice are as indicated. Scale bar ¼ 50 mm. inhibitors, disheveled, providing an additional Wnt- related mechanism by which Sirt1 may promote ...
Transfected HCT116 and SW620 cells (~1 × 107) were collected using non-enzymatic cell dissociation solution (Sigma-Aldrich), resuspended in 1 mL staining buffer (Miltenyi Biotec, Bergisch Gladbach, Germany), and then incubated with mouse anti-human CD44-FITC antibody a...
Anti-Hic-5 antibody specifically pulled down endogenous Smad1 in both the PC3 human prostate cell line and primary cultures of rat prostate fibroblasts, supporting that Hic-5 binds to Smad1 at the endogenous level. Bacterially expressed glutathione S-transferase (GST)-Smads 1, 5 or 8, but ...
Inhibition of TGF-β1 with a specific antibody in HTSF resulted in a statistically significant decrease in the expression of p21cip1 and p15ink4b compared to HTSF–TGF-β1 (2- and 14-fold, respectively). These results indicated that TGF-β1 controls the expression of cell-cycle proteins; ...