Cell Line Name: HEK293/hERGCat#: NTCC597625Gene Synonyms: KCNH2; ERG1; HERG; HERG1; Kv11.1; LQT2; SQT1Expressed Gene:...
hERG Recombinant Cell LinePlace, ToOrder, An
最早,分别采用爪蟾卵母细胞和中国地鼠卵巢细胞(CHO)转染hERG基因表达钾通道电流,发现前者比后者表达的钾电流要高[6-7];同时,这两种细胞都为异源性细胞,和人细胞还存在着种族的差异。因此,1996年Snyders等首次提出采用HEK293细胞可以稳定地表达hERG基因[8],如今已成为可靠的体外评价...
HERG基因转染HEK293细胞 及其编码通道电流的记录; l: 邝素娟 林吉进,2j 林曙光 邓春王 余细勇 吴书林 单志新 杨敏 [摘要] 目的 建立HERG基因在HEK293细胞稳定表达的方法。方法 利用Lipofectamine2000将pCDNA3.0一 HERG转染进入HEK293细胞,通过G418筛选阳性克隆细胞系,采用免疫荧光细胞化学方法检测该蛋白的表达,用 ...
Cell LineCHO-hERG cells; HEK293-hERG cells MethodElectrophysiology Test Compound ConcentrationAs required by the client Number of ReplicatesAs required by the client QCSeal resistance > 50 MOhms Pre-compound current ≥ 0.1 nA DeliverablesIC50; Report ...
因此,1996年Snyders等首次提出采用HEK293细胞可以稳定地表达hERG基因[8],如今已成为可靠的体外评价方法被广泛应用。 5)hERG抑制体外评价技术及影响因素 膜片钳技术被称为研究离子通道的“金标准”,是研究离子通道的最重要的技术。 随着研究的不断深入,目前膜片钳技术已从常规膜片钳技术...
We have established stably transfected HEK 293 cell lines expressing high levels of functional human ether-a go-go-related gene (HERG) channels. We used these cells to study biochemical characteristics of HERG protein, and to study electrophysiological and pharmacological properties of HERG channel cur...
参考文献 [1]. Yu D, et al. Inhibitory effects and mechanism of dihydroberberine on hERG channels expressed in HEK293 cells. PLoS One. 2017 Aug 1;12(8):e0181823. 所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务发表在 研究领域 | 标签为 dihydroberberine、herg、抑制剂 ...
Properties of HERG Channels Stably Expressed in HEK 293 Cells Studied at Physiological Temperature We have established stably transfected HEK 293 cell lines expressing high levels of functional human ether-a go-go-related gene (HERG) channels. We used th... Z Zhou,Q Gong,B Ye,... - 《Bioph...
cells, FAK is cleaved by caspase 3 upon treatment with doxazosin, which leads to apoptosis or anoikis (i.e. apoptosis due to loss of cell adhesion).67 Furthermore, hERG1, integrin b1, and FAK form a macromolecular complex in hERG1-transfected HEK293 cells and SH-SY5Y neuroblastoma cells....