Some functional testing can be accomplished in cultured cells such as HEK293 cells, but HEK293 cells limit the types of rAAV constructs that can be tested. Many rAAV constructs are designed to study neurons in the brain with neural-specific promoters and many are floxed with loxp sites to ...
A stable HEK cell line was created with CYP1B1 expression under the control of tetracycline to facilitate regulated and titratable expression (see the Methods section for more details). A cell line was also created for CYP1A1 as a counter screen to evaluate selectivity. CYP1A1 is the closest...
Only GFP + cell bodies that were completely filled with myc immunolabeling were considered to be myc + . The experimenter was blinded as to the experimental condition during quantification. In vitro electrophysiology A stable line of HEK cells expressing Nav1.3 and Kir2.1 (Ex-HEK30, ...
(c) Quantification of maximal calcium fluorescence of HEK293 cells expressing Magneto2.0 and subjected to the above conditions using Fluo-4 calcium imaging 24 hours post-transfection. Values shown are the average maximal Fluo-4 fluorescence values per cell relative to baseline for each condition. ...
All cell lines tested mycoplasma-negative. HEK293 and HEK293T (immortalized human female kidney-derived cells) were kind gifts from Marc Diamond lab (UT Southwestern). HeLa (immortalized human female cervical cancer-derived cells) were obtained from ATCC. U2OS cells (human female osteosarcoma cells...
Results In using the suspension-adapted human embryonic kid- ney (HEK) 293-F cell line, a modified serum-free Free- Style™ medium platform plus transfection by the 293- Free™ reagent, we were able to install a TGE shaker fermentation process with product yields of up to 200 mg/L ...
OBJECTIVE To establish a quality control method for anti-DLL3 monoclonal antibody-MPVP-PBD based on cysteine conjugation. METHODS The cell line HEK 293 T transfected with DLL3 was used as the target cells to determine the biological activity against of anti-DLL3 McAb-MPVP-PBD,and the binding...
TRPC6 overexpressing HEK-293 cells is more sensitive to oxidative insults and has larger pools of mobilizable Zn2+. Live cell-imaging experiments and whole-cell patch-clamp recordings show that TRPC6 channels, but not TRPC3 channels, can transport Zn2+ ions and this TRPC6-dependent Zn2+ ...
293 Control cell line Add to favorite 3-7 x 10e6 cells 293-null Please contactour distributor 293XL/null 293XL Control cell line Add to favorite 3-7 x 10e6 cells 293xl-null Please contactour distributor HEK293 Control cells 293/Control cellswere generated by stably transfecting HEK293 cell...
High titer lentivirus was produced. Briefly, HEK 293FT cells were plated to 90% confluence in a 4-layer cell factory (Nunc) cultured with DMEM containing 10% FBS. Cells were co-transfected with 690 μg of the lentiviral vector described above and two helper plasmids (690 μg of pΔCMVR...