正链RNA然后和核心蛋白相接触并被包裹为核心颗粒。此颗粒向内质网出芽形成病毒颗粒。病毒颗粒经高尔基体由分泌途径出胞。 看来E2蛋白也不仅仅是一个结构蛋白,它可能是HCV对干扰素的抗性的原因之一 (图3):E2被发现与INF诱导的蛋白激酶PKR(double-RNA activated protein kinase)有结合。后者催化转录起始因子eIF2-alpha的...
通过和其他RNA病毒相比较,推测这个高度保守的98碱基序列可能和病毒基因组的包装有关系,也可能参与RNA合成起始,或对两者都很重要。从具有感染力的cDNA克隆及其3'UTR的缺失突变株制备RNA转录本,用猩猩来测试感染性,发现多聚U、多聚C(U)或X区域的部分或全部缺失都使RNA转录本丧失了感染性;而破坏了5'端多变区2个茎...
months, your doctor will order a test called a qualitative nucleic acid test (NAT) to look for the virus currently in your blood. This measures the number of viral RNA (genetic material from the hepatitis virus) particles in your blood. They usually show up 1-2 weeks after you’re ...
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Hepatitis C virus (HCV) is a positive-strand RNA virus that causes significant pathology in humans. Here, Lindenbach and Rice discuss recent insights into the unique properties of HCV particles and then review HCV entry and assembly, with a focus on the
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The quantification of hepatitis C virus (HCV) is essential for the management of chronic hepatitis C therapy. We have developed a fully automated microfluidic RT-qPCR system for rapid quantitative detection of HCV RNA in human EDTA-plasma and serum, and
通过对捐献的血液进行HCV特异性抗体RNA筛查,我们已经基本上消灭了通过输血发生的HCV传播11。尽管如此,在2015年,全球仍有3%的输血未经过适当筛查。2030年的最终目标是100%筛查和消灭输血相关传播,扩充资源(包括低成本、高灵敏度测试)可能有助于低收入地区实现这一最终目标12。
Hepatitis C virus (HCV) RNA replication occurs in the cytosol of infected cells within a specialised membranous compartment. How the viral non-structural (NS) proteins are associated and organised within these structures remains poorly defined. We employed a super-resolution microscopy approach to visu...