HCV-RNAQUANTITATIVE DETECTIONMULTICYCLIC RT-PCRSALI SITE MUTANT CRT-PCRWe have developed a method to quantitate hepatitis C virus (HCV)-RNA from serum by using 'multicylic' RT-PCR. This method requires 100 渭l of sera and synthetic HCV-RNA with a known copy number to be used as a ...
hepatitis C virus RNA, quantitative by TaqMan PCR 中文名: 中文别名: CBNumber: CB910079149 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 hepatitis C virus RNA, quantitative by TaqMan PCR化学性质 安全信息 hepatitis C virus RNA, quantitative by TaqMan PCR性质、用途与生产工艺...
hepatitis C virus RNA, quantitative by real-time PCR| 基本信息 更多信息 中文名称: 中文同义词: 英文名称: hepatitis C virus RNA, quantitative by real-time PCR 英文同义词: hepatitis C virus RNA, quantitative by real-time PCR CAS号: 分子式: 分子量: 0 EINECS号: 信息错误报告 您的...
The quantification of hepatitis C virus (HCV) is essential for the management of chronic hepatitis C therapy. We have developed a fully automated microfluidic RT-qPCR system for rapid quantitative detection of HCV RNA in human EDTA-plasma and serum, and the performance of the method was assessed...
The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients. 展开 关键词: qPCR Hepatitis B Hepatitis C
As indicators to determine the diagnosis, condition, and prognosis of hepatitis C, methods have been developed such as the detection of HCV-RNA by the nested RT-PCR method, quantitative assay of HCV-RNA by the competitive RT-PCR method, qualitative and quantitative assay of various anti-HCV Ig...
C, replicase activity in the absence of inhibitor; 3dC, replicase activity in the presence of 7.5 μM 3′-dCTP. Table 1. Inhibition of HCV replicase and recombinant NS5B RNA synthesis activity by nucleotide and non-nucleoside inhibitors RNA synthesis assayIC50 [μM]a 3′-dCTPCompound 1...
We then went on to test whether other essential vi- ral replication steps were accomplished in C/OTg liver. Quantitative RT-PCR analysis of intracellular HCV RNA showed that HCV efficiently replicated in PHTTg cells (Figure 3A), although less robustly than in Huh7.5.1 cells, which are ...
MicroRNA Quantitative Real-Time PCR (Q-PCR) Assay Huh7.5.1 cells or cells infected with HCV were vortexed to lyse. Human liver biopsies were lysed in TissueLyser LT bead mill (Qiagen). Total miRNA was isolated using a miRNeasy Mini Kit per manufacturer's instructions (Qia- gen). RNA was...
Quantitative PCR assays used to monitor hepatitis B virus (HBV) load differ in their ability to detect different HBV variants. This study evaluated the per... V Thibault,S Laperche,S Akhavan,... - 《Journal of Virological Methods》 被引量: 19发表: 2009年 加载更多来源...