Nearly 60 million people worldwide are infected with Hepatitis C Virus (HCV), a bloodborne pathogen which leads to liver cirrhosis and increases the risk of hepatocellular carcinoma. Those with limited access to healthcare resources, such as injection dr
The study of serum specimens from patients infected with HCV genotypes 1 to 6 showed a satisfactory relationship between HCV RNA levels measured by the Aptima HCV Quant Dx assay, and both real-time PCR comparators (Abbott RealTime HCV and Cobas AmpliPrep/Cobas TaqMan HCV Test, version 2.0, ...
with the necessary oligonucleotide primers to transcribe the desired RNA sequence. The process produces complementary DNA (cDNA) which then undergoes PCR. During the PCR process the cDNA is incubated with a thermostable polymerase and primers to initiate the desired sequence. The solution then undergo...
对于基因型1b抗性选择,复制子细胞在asunaprevir存在下维持EC50值的10或30倍(分别为30或90nM终浓度)[3]。 Asunaprevir以200至600毫克的单剂量或多剂量每日两次给药,通常耐受性良好,慢性感染基因1型HCV的受试者的HCV RNA水平迅速大幅下降[4]。 In VivoAsunaprevir(ASV,3-15mg / kg,po)在几种动物物种中显示出...
Analysis of the viability of freezer stored serum samples for hepatitis C virus RNA analysis by the SUPERQUANT method: results of a 16 year retro- ... P Schmid,M Tong,A Conrad,... 被引量: 0发表: 1999年 The 1999 Chromosome Committee Reports for the Mouse Genome: Introduction Prior to ...
Analysis of the viability of freezer stored serum samples for hepatitis C virus RNA analysis by the SUPERQUANT method: results of a 16 year retrospective study. J Virol Methods 1999; 82: 201-6.Schmid P, Tong M, Conrad A, McHutchison J, Blatt LM. Analysis of the viability of freezer ...
The Cobas AmpliPrep/Cobas TaqMan HCV test,version 2.0,real-time PCR assay accurately quantifies hepatitis C virus genotype 4 RNA. Chevaliez S,Bouvier-... S Chevaliez,M Bouvier-Alias,C Rodriguez,... - 《Journal of Clinical Microbiology》 被引量: 30发表: 2013年 The Cobas AmpliPrep/Cobas ...
Reverse transcription was performed with 2 μg of total RNA for all genes with the cDNA synthesis kit (Cat. 4368814). Using 240 ng of random primer, 2U RNAse inhibitor, 5 mM of DTT, 1 mM of dNTPs and 200U of transcriptase, PCR was performed using the following protocol: 65°C/5 ...
1a), whereas reverse transcription (RT)–PCR of two livers confirmed the presence of AAV2 mRNA from the Cap open reading frame (Extended Data Fig. 1c). In the blood samples, which had not been treated to preserve RNA, we detected low levels of AAV2 RNA reads mapping throughout the ...
In PLC/PRF/5 cells, the cccDNA PCR amplifies integrated HBV DNA (a segment containing the promoter for pgRNA). In these cells which contain multiple integrations of the S region, the pgRNA/S-RNA ratio was low (C). In Huh7.5 cells, the cccDNA levels, pgRNA per cc...