GastroenterologyHeintges T, Mohr L, Niederau C, et al. Quantitative hepatitis C RNA-polymerase chain reaction and detection with DNA- ELISA. Hepatogastroenterology 1998;45:1684-1689.Heintges et al. " Quantitative hepatitis C RNA-polymerase chain reaction and detection with DNA-ELISA " Hepato...
Hepatitis C Virus(HCV)丙型肝炎病毒4型RT-PCR试剂盒 运输:低温 保存:负20度 有效期:一年 货期:2-3周 产品及特点 1.即开即用,用户只需要提供DNA/RNA样品。 2.根据丙型肝炎病毒4型保守序列设计的专一性引物,与相关病毒无交叉反应。 3.灵敏度可以达到一千拷贝/反应。
Hepatitis C virus (HCV)-RNA in non-A, non-B chronic hepatitis in France. Nucleotide sequence of a French HCV isolate. J Hepatol. 1991; 13 (Suppl 4):S24–S32.Kremsdorf D, Porchon C, Brechot C (1991): Hepatitis C virus (HCV)- RNA in non-A non-B chronic hepatitis in France. ...
产品名称:HepatitisBVirusPregenomicRNA(HBVpgRNA)乙型肝炎病毒前基因组RNA探针法荧光定量RT-PCR试剂盒 规格:50T 品牌:森兴研 PCR(Polymerase Chain Reaction)是一种常用的分子生物学技术,可以在体外迅速扩增目标DNA片段。PCR技术的应用广泛,包括基因定量分析、遗传诊断、病原微生物检测等。PCR检测试剂盒操作步骤是进行PCR...
Flgure3 Ethidiumbromide staining of agarose gel showing amplification products of hepatitis C virus RNA from serum samples by ligation- dependent polymerasechain reaction (LD-PCR). Expected size of PCR product (102 base pairs) is indicated by arrow. Lanes 1 and 2, uninfected; lanes 3-6, infe...
As indicators to determine the diagnosis, condition, and prognosis of hepatitis C, methods have been developed such as the detection of HCV-RNA by the nested RT-PCR method, quantitative assay of HCV-RNA by the competitive RT-PCR method, qualitative and quantitative assay of various anti-HCV Ig...
The quantification of hepatitis C virus (HCV) is essential for the management of chronic hepatitis C therapy. We have developed a fully automated microfluidic RT-qPCR system for rapid quantitative detection of HCV RNA in human EDTA-plasma and serum, and
The cells were washed with PBS and then bound virions were determined by analyzing HCV RNA levels by qRT-PCR. (Right panel) Huh7.5 cells were transfected with the indicated siRNAs. At 48 h after transfection, cells were incubated with Jc1 at 4 °C for 2 h. The cells were ...
The RNA concentration of the samples was determined from the standard curve generated using the ribosomal RNA standards. Real-time qRT-PCR assays The “TaqMan EZ RT-PCR kit” (Applied Biosystems; ABI) was used to perform RT-PCR reactions on a 7500 Real Time PCR System (ABI). For the ...
DETECTION OF MINUS STRAND RNA OF HCV IN LIVER TISSUES OF PATIENT WITH HEPATITIS C AND HEPATOCELLULAR CARCINOMA BY RT-PCR 11 cases of drug addicts with their serum anti-HCV positive and 10 cases of hepatocellular carcinoma were examined for HCV genes (plus strand RNA) and HCV ... C Zhi,H...