Nonreactive (negative). This means you're not currently infected with hep C. If you think you've been exposed within the last 6 months, you will need to be tested again to make sure because it can take a while for your antibody levels to rise high enough to be detected on a test....
Nonreactive (negative). This means you're not currently infected with hep C. If you think you've been exposed within the last 6 months, you will need to be tested again to make sure because it can take a while for your antibody levels to rise high enough to be detected on a test....
meaning to be unaffected by oxygen tension (despite the name HIF-1β).1,4,14 In addition, it is assumed that ARNT is available in excess inside the cell.15 However, there is accumulating evidence that certain cell lines are capable to upregulate ARNT in hypoxia,16–18 which implies a ...
For cytoskeletal proteins, either a rabbit anti-b-tubulin (diluted 1:10 in 3% BSA in PBS and revealed by a Texas red-conjugated anti-rabbit-IgG antibody) for 1 h or a 10 mM FITC-conjugated phalloidin for 20 min were used. All the incubations were performed at room tem- perature. ...
HepG2 cells were subsequently incubated with anti-CTR1 antibody and Alexa-488 anti-rabbit antibodies (1:5000) sequentially for 1 h in room temperature. The fixed cell images were recorded using a Leica TCS SP8 Confocal Microscope System (405 nm and 488 nm lasers), at 63 × ...
Thereafter, blots were incubated for 1 h with the secondary antibody conjugated to horseradish peroxidase (1:5000). Immunoreactive bands were visualized by detection of peroxidase activity by DAB staining (0.05% DAB in TBS + 10 μL 30% hydrogen peroxide). The analysis of the obtained results ...