Nonreactive (negative). This means you're not currently infected with hep C. If you think you've been exposed within the last 6 months, you will need to be tested again to make sure because it can take a while for your antibody levels to rise high enough to be detected on a test....
Nonreactive (negative). This means you're not currently infected with hep C. If you think you've been exposed within the last 6 months, you will need to be tested again to make sure because it can take a while for your antibody levels to rise high enough to be detected on a test....
The kit also includes buffers for Compound Dilution, Fixation, Cell Permeabilization, Antibody Dilution and Washing, as well as disposable syringes and needles for HepG2 cell handling and plate sealers. All materials and protocols provided have been validated for multiplexed analysis on both the GE ...
The sample was added to a 10% gel for electrophoresis. After electrophoresis, the protein was transferred to the membrane (Millipore, #IPVH00010) and then hybridized with the antibody. Finally, the signal on the membrane was detected by an ECL kit (Thermo, #34096). Antibodies: GPX4 (Abcam...
Several studies have estab- lished that hydrazine in response to its easy oxidation to chemically reactive species causes protein denaturation, induces DNA damage, which leads to damage in hepatic system. The Asialoglycoprotein receptor (ASGPR) is highly expressed on the surface of hepatocytes. ...
A total of 254 mRNAs were identified as up- or down-regulated over 1.5- fold in Akbu-LAAO-treated HepG2 cells in comparison with control HepG2 cells. Among these targeting genes, we focused on the molecules involved in TGF-β signal pathway. Gene-gene interaction network analysis ...
Fe-SOD was purified from bacterial lysate by Ni-NTA column chromatography and used to generate an anti-SOD antibody. The purified Fe-SOD was encapsulated in liposomes and delivered to HepG2 liver tumor cells to eliminate cellular superoxide anions. The SOD-loaded cells exhibited lower reactive ...
A total of 100 μL of, respectively, the various ECM proteins solution and HepG2-CM was pipetted in triplicate in the wells for immobilization at 4 °C, overnight. Wells treated with 1× PBS or HepG2-CM were used as controls. After overnight incubation, the wells were carefully ...
A total of 21 proteins were identified as differentially expressed proteins and these 21 proteins were all up-regulated by Arg supplementation. Six amino acid metabolism-related proteins, mostly metabolic enzymes, showed differential expressions. Intriguingly, Ingenuity Pathway Analysis (IPA) based pathway...
The redox state of TRX1 was detected by western blotting with anti-TRX1 antibody. (C) Isolated mitochondria were pre-incubated with 4 µg/ml oxidized rTRX1, then As2O3 was added at the indicated time (200 s). Trace a, Control (CaCl2 added at 1 800 s); Trace b, 40 µM As2O...