Exosomes from HEK293T cells small RNA-seq readsShurtleff, Matthew JKarfilis, Kate VTemocheDiaz, Morayma MRi, SayakaSchekman, Randy
In this study, RNA-Seq data obtained from single HEK293T cells have been used to analyze expressions and functions of heterogeneous circRNA profiles. The enrichment patterns of circRNAs, interactions with miRNAs and pathways such as ErbB signaling pathway and protein processing in endoplasmic reticulum...
A novel transcript (NR5A2-KLHL29FT) was identified by RNA-seq and validated in colonic tissue samples. NR5A2-KLHL29FT is transcribed from a polymorphic insertion at the KLHL29 locus, rather than a chromosomal rearrangement between chromosomes 1 and 2. This polymorphic insertion does not appear...
因此,构建KDM2A的敲除细胞系,并以此用于细胞凋亡信号通路的研究是极为重要的,构建成功的细胞系可用于蛋白水平表达、转录组分析以及其他多种实验用途。虽然目前有部分关于KDM2A基因沉默以及RNA干扰的相关报道,但是与基因敲除相比,基因敲除的效果更为彻底,更有利于研究KDM2A对细胞增殖和凋亡作用的影响。
rRNA-depleted RNA-Seq analysis of HEK-293T cells.Harm, van BakelCorey, NislowBenjamin, J. BlencoweTimothy, R. Hughes
Through the RNA-seq analysis of the differential expressed genes between KDM2A knockdown HEK293T cells and wild type, we screened out that TGF-β pathway was significantly downregulated in KDM2A knockdown cells, which indicates that TGF-β signaling pathway might be the downstream target of KDM2A...
3.4 RNA 测序和数据处理 RNA-Seq 和数据处理 RNA-seq 的执行如前所述。进行 RNA-seq 来比较 pCAGGS-HA-A5L 转染的 293T 细胞和 pCAGGS-HA(空载体)转染的 293T 细胞在 24 小时内的基因表达谱。 从每组中随机选择三个样本来制备RNA-seq文库。使用 Trizol 试剂从每个样品中提取总 RNA。 使用 NanoDrop 分光...
RNA-seq analysis identified a total of 1473 differentially expressed genes (DEGs), comprising 911 upregulated and 562 downregulated genes. Additionally, LC-MS/MS analysis revealed 185 cellular proteins with significantly altered abundance ratios that interact with the A5L protein. Here, we perform ...
A total of 648 differentially expressed genes (DEGs) were identified in cells by RNA-Seq, including 314 upregulated genes and 334 downregulated genes. Additionally, liquid chromatography–tandem mass spectrometry (LC-MS/MS) detected 115 cellular proteins that interact with the A23 proteins. ...
2.7. RNA-seq and KEGG pathway enrichment analysis Two groups of cells were collected: 293T cells (two replicates) and NDUFS7-deficient 293T cells (two replicates). Total RNA was extracted from cells using TRIzol reagent and treated with DNase I to remove genomic DNA. After the assessment of...