Today, HEK293 cells are frequently used in cell biology and biotechnology, second only to HeLa, the first human cell line. Around establishment of HeLa in 1951, scientists were reluctant to accept and use human cell lines out of concern for an oncogenic agent in them. This concern, along wi...
The 293 cell line was derived by transformation of primary cultures of human embryonic kidney (HEK) cells with sheared adenovirus (Ad)5 DNA. A combination of immunostaining, immunoblot, and microarray analysis showed that 293 cells express the neurofilament (NF) subunits NF-L, NF-M, NF-H, ...
Three distinct HEK-293 cell lines expressing non-toxic proteins; voltage gated calcium channels called Cav3.1, Cav3.2, or Cav3.3. Highlights: Express low voltage-activated (LVA), T-type, Ca2+ Channel (Cav) 3.1, 3.2, or 3.3 All cDNAs of human origin Cav3.x expression is under G418 ...
Cell type:Epithelial Tissue origin: Human Embryonic Kidney Target:TGF-β Specificity:Human, mouse Reporter gene:SEAP Antibiotic resistance:Zeocin® Detection range:10 pg/ml - 10 ng/ml (hTGF-β and mTGF-β) Growth medium:Complete DMEM (see TDS) ...
293-mtlr2 Please contactour distributor HEK293 clones expressing TLR2 293/TLR2 cellswere obtained by stable transfection of HEK293 cells with a pUNO-TLR2 plasmid which expresses the human or murine TLR2 gene. The control cell line for 293/hTLR2 and 293/mTLR2 cells is293/null. ...
and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin. ...
6). We recently demonstrated that CI inhibition triggers cell death in melanoma cancer cell lines by a mechanism involving ROS and opening of the mPTP [1]. Here we observed that 2 h pre-treatment with 0.5 µM cyclosporin A (CsA), which reduces the mPTP open probability [2], inhibited...
The HEK293 derived suspension cell lines 293-F and Freestyle 293-F (Thermo Fisher Scientific) have been used in this study to generate stable cell clones expressing GFP or EPO. Method details Cell line generation and cultivation HEK293 cell lines 293-F and Freestyle 293-F were cultivated in...
Generation of a stable IFNα2b-expressing HEK293 cell clone and production in fed-batch cultures The expression plasmid pYD7 encoding the human IFNα2b gene codon-optimized for expression in human cells (Fig. 1A) is derived from the previously described pTT vector [37]. The signal peptide seq...
amino groups, the adhesion observed is not integrin-dependent and is instead based on enhancing electrostatic interactions between the coated surface and the negatively charged residues in the cell membrane18. This interaction seems to override 5-HT2A-mediated adhesion in HEK293-based cell lines. ...