早期报道中指出该细胞基因组中含有腺病毒5(Ad5)基因组的左侧端和右侧端的DNA,但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现,Ad5的1～4344位线性核苷酸整合入细胞染色体19q13.2。HEK293细胞-人胚胎肾细胞为人类腺病毒载体扩增的宿主。可表达异常的玻连蛋白的细胞表面受体,由整合素β1亚单位...
We have established stably transfected HEK 293 cell lines expressing high levels of functional human ether-a go-go-related gene (HERG) channels. We used these cells to study biochemical characteristics of HERG protein, and to study electrophysiological and pharmacological properties of HERG channel cur...
In our attempt to optimize plasmid ratios to enhance fully packaged rAAV particle yield, we discovered cell-line-specific optimization potential. The findings highlight the transfection's complexity, urging tailored strategies for improved rAAV production based on each cell line's characteristics, ...
The productions with parental cell lines (HEK293SF and 293SF-CymR/λR-GyrB) were done with standard triple plasmid transfection as well as five plasmid transfections containing plasmids encoding Rep expression cassettes used to create the stable cell lines (Figure 4A). We observed that functional...
Cell Lines HEK293 Cells HEK293 TLR Reporter Cells Mouse TLR4 Reporter HEK293 Cells (NF-κB)Research Fields Products HEK293 Cells HEK293 TLR Reporter Cells TLR2 reporter cells HEK-Blue™ hTLR2 HEK-Blue™ hTLR2 KO-TLR1/6 HEK‑Blue™ hTLR2-TLR1 HEK‑Blue™ hTLR2-TLR6 HE...
Cell Lines HEK293 Cells HEK293 TLR Reporter Cells Human MD2 and CD14 Reporter HEK293 Cells (NF-κB)Research Fields Products HEK293 Cells HEK293 TLR Reporter Cells TLR2 reporter cells HEK-Blue™ hTLR2 HEK-Blue™ hTLR2 KO-TLR1/6 HEK‑Blue™ hTLR2-TLR1 HEK‑Blue™ hTLR2...
(RT-PCR), and RNA sequencing and invasion assays.OMUL-1-an adherent cell line-resulted in 100% tumor formation when subcutaneously injected into mice. Histological analysis of the subcutaneous tumor using hematoxylin and eosin staining revealed squamous cell carcinoma with characteristics similar to ...
of isogenic, inducible, stable cell lines and permits polyclonal selection of these cell lines. Once the Flp-In T-REx host cell line containing an integrated FRT site has been created, subsequent generation of Flp-In T-REx cell lines expressing the gene(s) of interest is rapid and efficient...
transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expressi...
FAQ Cell Lines 96 /100 333 Citations Related products Notification: Reference #hkb-htlr4-av can only be ordered together with reference #hkb-htlr4.NF-κB–SEAP reporter HEK293 cells expressing human TLR4Signaling pathways in HEK-Blue™ hTLR4 cells (click to enlarge and see legend) HEK...