The authors reported CRISPR/Cas9 technique as an efficient and reliable tool for developing stable cell lines by site-specific integration.Dissecting the roles of GRK2 and GRK3 in μ-opioid receptor internalization and β-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells Scientific Reports ...
Available cell lines: IP3R null, IP3R1 null, IP3R2 null, IP3R3 null, IP3R1+, IP3R2+, IP3R3+ IP3R null HEK-293 created via CRISPR/Cas9 technology, targeting the ITPR1, ITPR2, and ITPR3 genes IP3R null cells do not respond to extracellular stimuli which would normally raise intrac...
Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production Biotechnology Letters The authors reported CRISPR/Cas9 technique as an efficient and reliable tool for developing stable cell lines by site-specific integration. Dissecting the roles...
Transfection reagent for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells
Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production Biotechnology Letters The authors reported CRISPR/Cas9 technique as an efficient and reliable tool for developing stable cell lines by site-specific integration. ...
To investigate the transcriptional regulation of the PGRN gene, a luciferase knock-in reporter system was established in HEK293 cells by integrating luciferase gene in the genome controlled by the endogenous PGRN promoter using CRISPR/Cas9. PCR results demonstrated the site-specific integration of the...
CRISPR/Cas9CelllineKnock-inSREBP1TranscriptionalregulationTo establish a HEK293 cell line with a luciferase knock-in reporter controlled by the endogenous SREBP1 promoter for investigating transcriptional regulation of the SREBP1 gene.PCR confirmed the site-specific integration of a single copy of the ...
L10494 RNF26基因敲除HEK293T细胞Trizol裂解液 500µl 1258.00元RNF26 Knockout HEK293T Cells (RNF26基因敲除HEK293T细胞)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因,并实现了目的基因CRISPR敲除的HEK293T细胞。本细胞中目的基因的敲除已经通过T7EI法的验证。本细胞株是多克隆细胞,可用于该目的基因的...
Generation of cPKC and nPKC KO HEK293A cell lines To investigate GPCR regulation by PKC in more detail, PKC KO cell lines were generated via CRISPR/Cas9-mediated gene editing. The human genome encodes eight second messenger–sensitive PKC isozymes, including two splice variants of the PRKCB gen...
RASAL3 Knockout HEK293T RIPA Lysate(RASAL3基因敲除HEK293T细胞RIPA裂解液)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因并实现了目的基因CRISPR敲除的多克隆HEK293T细胞的RIPA裂解液。该细胞中目的基因的敲除已经通过T7EI法的验证。本产品可用于该目的基因敲除后其信号通路相关蛋白的研究,也可以用于该基因相...