The authors reported CRISPR/Cas9 technique as an efficient and reliable tool for developing stable cell lines by site-specific integration.Dissecting the roles of GRK2 and GRK3 in μ-opioid receptor internalization and β-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells Scientific Reports ...
Available cell lines: IP3R null, IP3R1 null, IP3R2 null, IP3R3 null, IP3R1+, IP3R2+, IP3R3+ IP3R null HEK-293 created via CRISPR/Cas9 technology, targeting the ITPR1, ITPR2, and ITPR3 genes IP3R null cells do not respond to extracellular stimuli which would normally raise intrac...
背景资料 Cell Lines产品描述 ACTB CRISPR 稳定 HEK293T 细胞系,Kidney-Excretory System产品形式 细胞生物学保存建议 Vapor phase of liquid nitrogen, or below -130其他 Applied Biological Materials Inc(abm)公司是加拿大一家不断寻找用于生命科学研究和药物开发新产品的公司。众多的产品线全面涵盖了许多尖端技术,...
Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production Biotechnology Letters The authors reported CRISPR/Cas9 technique as an efficient and reliable tool for developing stable cell lines by site-specific integration. Dissecting the roles...
CRISPR/Cas9HEK 293细胞Rev-erbβ基因敲入基因敲除目的 利用成簇的,规律间隔的短回文重复序列/Cas9核酸酶(CRISPR/Cas9)基因组编辑技术,构建Rev-erbβ基因敲除的HEK293细胞系.方法 通过单向导RNA (sgRNA)介导Cas9蛋白对目的基因靶位点DNA进行特异性的切割,然后经DNA同源重组单向导RNA或非同源末端连接...
ROS1 Knockout HEK293T RIPA Lysate(ROS1基因敲除HEK293T细胞RIPA裂解液)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因并实现了目的基因CRISPR敲除的多克隆HEK293T细胞的RIPA裂解液。该细胞中目的基因的敲除已经通过T7EI法的验证。本产品可用于该目的基因敲除后其信号通路相关蛋白的研究,也可以用于该基因相应抗...
L03764MLLT11基因敲除HEK293T细胞Trizol裂解液500µl1258.00元 MLLT11 Knockout HEK293T Cells (MLLT11基因敲除HEK293T细胞)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因,并实现了目的基因CRISPR敲除的HEK293T细胞。本细胞中目的基因的敲除已经通过T7EI法的验证。本细胞株是多克隆细胞,可用于该目的基因的生...
transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expressi...
2.1. Generation of Isogenic ARC-KO HEK293 Cell Lines via CRISPR/Cas9 Editing IsogenicARC-KO HEK293 cell lines were generated using CRISPR/Cas9 genome editing of HEK293 cell lines. First, guide RNA (gRNA) was designed to target unique sequences present in the 5′UTR-exon 1 ofARC(Figure 1A...
(Fig.2). We used serial dilution to separate single cell clones from heterogeneous cell populations to assess the function of the CRISPR/Cas9 system. Upon introducing the Cas9-gRNAs system into cells, we expected to see four potential cell lines: cell lines without mutation, with heterozygous ...