Quantitative detection of HCV RNA was performed by TaqMan real-time PCR. The results showed that no decrease in the concentration of HCV RNA was detected over the 14 days period at 4°C (P > 0.05) for the high and intermediate HCV RNA load groups. However, after 21 and 28 days of ...
For quantitative detection of HCV specific RNA using real-time RT-PCR Products Product Details Resources FAQ Products artusHCV RT-PCR Kits CE are intended for in-vitro diagnostic use. Features Complete CE-IVD-compliant workflow from sample to quantitative detection ...
real-time, for RT-PCR Description INOGene-Hepatitis C Virus (HCV) Quantitative RT-PCR Kit is an in vitro nucleic acid amplification assay for quantification of Hepatitis C virus (HCV) RNA in human serum or plasma (EDTA) using viral RNA extraction kits via Real-Time PCR instrument. INOGene...
Quantitative real-time PCR. Using the Transcriptor First-Strand cDNA Synthesis Kit (Roche Applied Science), cDNA was synthesized from 1 mg of total RNA. Quantitative PCR was performed using LightCycler 480 Probes Master (Roche Applied Science) on a LightCycler 480 (Roche Applied Science). Signals...
目前RQPCR检测HCV RNA在临床已广泛应用,RQPCR技术融合了PCR技术与DNA探针杂交技术的优点,实验的整个过程只在加样时打开1次反应管,在PCR的每个循环中可以直接监测到荧光信号的变化,根据PCR反应酶动力学特点,分析软件会自动对DNA进行定量,因此也有人称RQPCR为实时荧光定量PCR[6](realtime quantitative PCR)。其优点较多...
Quantitative real-time PCR was performed in triplicate as described previously4. Gene expression was normalized to GAPDH levels and is presented as fold changes using the 2-ΔΔct method. PCR primer sequences are shown in Table 1. Table 1 Primers sequences for quantitative RT-PCR Full size ...
Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method Methods, 25 (2001), pp. 402-408 View PDFView articleGoogle Scholar 39 M. Flint, et al. Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular...
2.1.1HCV RNA 检测方法采用的是实时定量PCR(Quantitative Real-time PCR)的方法;所用仪器为美国AB7500PCR实时荧光定量PCR仪;试剂厂商为中山大学达安基因股份有限公司。定量线性范围:5×102-108IU/mL;小于5×102IU/mL结果为阴性。大于5×102IU/mL结果为阳性,报具体定量数值结果。实验严格按照说明书进行,每天开展室内...
1.1.2.2采用荧光定量聚合酶链反应(FQ-PCR)法来检测患者血清中HCVRNA的含量,检测仪器系美国ABI公司7500RealTimePCRSystem检测仪,检测试剂盒来自凯杰生物工程(深圳)有限公司,结果以IU/mL表示,结果判定:≥5.0×102IU/mL为阳性。检测实验室为卫生部临床检验中心技术认可的临床基因扩增检验实验室。
【摘要】目的 探讨丙型肝炎病毒(hepatitis C virus,HCV)载量水平与HCV基因型别的相关性.方法 选取255例丙型肝炎患者,采用实时荧光定量逆转录PCR(reverse transcription quantitative real time PCR,RT-qPCR)检测HCV病毒载量水平,采用PCR-反向点杂交法(polymerase chain reaction-reverse dot blot,PCR-RDB)进行HCV基因...