Quantitative detection of HCV RNA was performed by TaqMan real-time PCR. The results showed that no decrease in the concentration of HCV RNA was detected over the 14 days period at 4°C (P > 0.05) for the high and intermediate HCV RNA load groups. However, after 21 and 28 days of ...
2.1.1HCV RNA 检测方法采用的是实时定量PCR(Quantitative Real-time PCR)的方法;所用仪器为美国AB7500PCR实时荧光定量PCR仪;试剂厂商为中山大学达安基因股份有限公司。定量线性范围:5×102-108IU/mL;小于5×102IU/mL结果为阴性。大于5×102IU/mL结果为阳性,报具体定量数值结果。实验严格按照说明书进行,每天开展室内...
real-time, for RT-PCR Description INOGene-Hepatitis C Virus (HCV) Quantitative RT-PCR Kit is an in vitro nucleic acid amplification assay for quantification of Hepatitis C virus (HCV) RNA in human serum or plasma (EDTA) using viral RNA extraction kits via Real-Time PCR instrument. INOGene...
目前RQPCR检测HCV RNA在临床已广泛应用,RQPCR技术融合了PCR技术与DNA探针杂交技术的优点,实验的整个过程只在加样时打开1次反应管,在PCR的每个循环中可以直接监测到荧光信号的变化,根据PCR反应酶动力学特点,分析软件会自动对DNA进行定量,因此也有人称RQPCR为实时荧光定量PCR[6](realtime quantitative PCR)。其优点较多...
Real-time RT-PCR Confluent monolayers of replicon cells were lysed by Trizol (Invitrogen–Gibco) and RNA was precipitated with 75% EtOH and captured with a Micro RNeasy silica column (Qiagen, Venlo, The Netherlands). RNA was quantitated using a Nanodrop ND-1000 (Wilmington, DE, USA) and ...
Total RNA was isolated and analyzed by real-time RT-PCR for shelterin mRNA expression. Values were normalized to GAPDH mRNA and calculated relative to HS. c Shelterin protein expressions in naïve CD4 T cells isolated from HCV patients and HS. GAPDH is used as loading control. Representative ...
目前RQPCR检测HCV RNA在临床已广泛应用,RQPCR技术融合了PCR技术与DNA探针杂交技术的优点,实验的整个过程只在加样时打开1次反应管,在PCR的每个循环中可以直接监测到荧光信号的变化,根据PCR反应酶动力学特点,分析软件会自动对DNA进行定量,因此也有人称RQPCR为实时荧光定量PCR[6](realtime quantitative PCR)。其优点较多...
HIV and HCV RNA by quantitative PCR (Cobas TaqMan; Roche Diagnostics, Branchburg, NJ, United States) and HCV genotypes by a line probe assay (Versant HCV, Sie- mens). Routine laboratory methods were used to calculate three LF indexes: aspartate aminotransferase (AST) and platelets for APRI ...
HCV RNA水平与HCV基因型别的相关性研究 周萍;杨柳;李金洁;王晓琴 【摘要】目的 探讨丙型肝炎病毒(hepatitis C virus,HCV)载量水平与HCV基因型别的相关性.方法 选取255例丙型肝炎患者,采用实时荧光定量逆转录PCR(reverse transcription quantitative real time PCR,RT-qPCR)检测HCV病毒载量水平,采用PCR-反向点杂交...
HCV RNA replication was measured at various times post infection by real-time quantitative PCR as described above. Statistical analyses All statistical analyses were performed using GraphPad Prism software (GraphPad, San Diego, CA). All graphs represent the mean ± standard error of the mean (SEM)...