Real- time Quantitative PCR method was used to detect HCV-RNA , and at the same time liver function (ALT, AST) was detected. Results There were 95 HCV-RNA-positive cases from the 112 HCV-cAg positive specimens ( a positive rate of 84.8%) and 142 HCV-RNA-positive cases from 205 HCV-...
2007. Evaluation of the Abbott RealTime HCV assay for quantitative detection of hepatitis C virus RNA. J. Clin. Virol. 38 96-100. [ PubMed ]Michelin BD, Muller Z, Stelzl E, Marth E, Kessler HH (2007) Evaluation of the Abbott RealTime HCV assay for quantitative detection of hepatitis ...
2.1.1HCV RNA 检测方法采用的是实时定量PCR(Quantitative Real-time PCR)的方法;所用仪器为美国AB7500PCR实时荧光定量PCR仪;试剂厂商为中山大学达安基因股份有限公司。定量线性范围:5×102-108IU/mL;小于5×102IU/mL结果为阴性。大于5×102IU/mL结果为阳性,报具体定量数值结果。实验严格按照说明书进行,每天开展室内...
Objective To establish real-time fluorescence quantitative reverse transcription-polymerase chain reaction(RFQ-RT-PCR) for HCV RNA by using double-probe,and to resolve the problems of sensitivity and specificity of HCV RNA quantification.Method A total of 89 anti-HCV positive cases and 220 anti-HCV...
On the other hand, our previous observation [9] hints false negative anti-HCV results are becoming more frequent in immunocompromised patients, particularly those with AIDS. Real-time RT-PCR is an alternative strategy that does not have this disadvantage. However, detection of HCV-RNA by real-...
and detected by Real-time fluorenscent quantitative PCR. Results The results of HCV RNA in serum samples which were preserved 1 day and 1 week at room temperature,4℃and -20℃showed no significant difference. Conclusion Preserved temperature and preserved time have no effect on test results. 【...
HCV RNA load may be associated with the progress and severity of disease.Key words:HCV RNA; Double-probe; Fluorescence quantitative; Hepatitis C丙型肝炎病毒是引起输血后肝炎的重要病原之一,常呈无症状的持续感染,约65%的患者会发生肝硬化和肝癌1。丙型肝炎病毒核糖核酸(HCV RNA)实时荧光定量(RFQ-RT-PCR...
HCV RNA -(real-time PT-PCR) branched DNA PCR-hybridization . Real-Q HCV Quantification kit . : Real-Q HCV Quantification kit kit , , , , HCV RNA , Cobas Amplicor HCV Monitor 2.0 . : HCV RNA , . Hepatitis B virus Human Immunodeficiency virus , 9.5215.84%, 9.4017.53% (between-day...
HCV RNA was determined by quantitative realtime polymerase chain reaction (real-time PCR).The data obtained were divided into six groups according to the result of HCV RNA level.Then the correlation of serum HCV-RNA level with liver function indices and blood routine parameters was analyzed....
The EIA was compared with multiplex PCR method. RESULTS: The serotype 1 (Simmonds' classification, 1a, 1b, 1c), the most prevalent form in Korea followed by serotype 2 (Simmonds' classification, 2a, 2b, 2c). The distribution of serotypes and HCV viral loads were not different according ...