We analyzed 30 patients known to be positive with high/intermediate/low HCV RNA load, and kept their serum samples in storage at 4°C for 0, 1, 2, 3, 7, 14, 21 and 28 days. Quantitative detection of HCV RNA was
2A). The NS5b shRNA reduced HCV replication by 97 ± 2% (SEM) (P < 0.001) and IRES shRNA by 82 ± 8% (P < 0.05). The Con (10 ± 3%) and CD81 (20 ± 3%) shRNAs showed a slight reduction in HCV replication but it was not significant compared to mock control. Results ...
2.1.1HCV RNA 检测方法采用的是实时定量PCR(Quantitative Real-time PCR)的方法;所用仪器为美国AB7500PCR实时荧光定量PCR仪;试剂厂商为中山大学达安基因股份有限公司。定量线性范围:5×102-108IU/mL;小于5×102IU/mL结果为阴性。大于5×102IU/mL结果为阳性,报具体定量数值结果。实验严格按照说明书进行,每天开展室内...
RNA isolation and real-time RT-PCR Total RNA was extracted from 1 × 106 cells using PureLink RNA Mini Kit (Invitrogen, Carlsbad, CA), and complementary DNA was synthesized using High Capacity cDNA Reverse Transcription Kit (Applied Bio systems, Foster city, CA) per the manufacturer’s ...
Moreover, the study had an observational real-world nature and employed the retrospective data collection method, which may have possible bias, entry errors, and underestimation of side effects. We have not performed a quantitative assessment of HCV RNA, because in accordance with the requirements ...
and detected by Real-time fluorenscent quantitative PCR. Results The results of HCV RNA in serum samples which were preserved 1 day and 1 week at room temperature,4℃and -20℃showed no significant difference. Conclusion Preserved temperature and preserved time have no effect on test results. 【...
1.1.2.2采用荧光定量聚合酶链反应(FQ-PCR)法来检测患者血清中HCVRNA的含量,检测仪器系美国ABI公司7500RealTimePCRSystem检测仪,检测试剂盒来自凯杰生物工程(深圳)有限公司,结果以IU/mL表示,结果判定:≥5.0×102IU/mL为阳性。检测实验室为卫生部临床检验中心技术认可的临床基因扩增检验实验室。
HBV DNA和HCV RNA检测试剂抗干扰能力验证
HIV and HCV RNA by quantitative PCR (Cobas TaqMan; Roche Diagnostics, Branchburg, NJ, United States) and HCV genotypes by a line probe assay (Versant HCV, Sie- mens). Routine laboratory methods were used to calculate three LF indexes: aspartate aminotransferase (AST) and platelets for APRI ...
Quantitative real-time PCR. Using the Transcriptor First-Strand cDNA Synthesis Kit (Roche Applied Science), cDNA was synthesized from 1 mg of total RNA. Quantitative PCR was performed using LightCycler 480 Probes Master (Roche Applied Science) on a LightCycler 480 (Roche Applied Science). Signals...