A real-time PCR method for the quantitative analysis of HBV DNA in serum and liver tissuebenigncomplicationsesophagusstenosisstentExpandable metallic stents have become popular in recent years for the treatment
Here are the Steps for HBV DNA Quantitative Detection: Blood sampling for detection of hepatitis B virus DNA Detecting the hepatitis B virus DNA Observe the viral load. If the viral load is high, it indicates the HBV is actively replicated in the patient's body.DaAn...
Key words:Real time fluorescence quantitative PCR; Polymerase chain reaction; HBV-DNA; Quality control HBV DNA含量是诊断和监测乙型病毒性肝炎的重要指标[1]。目前测定HBV DNA含量的常用方法是实时荧光定量PCR法,想获得可靠的HBV DNA含量测定结果,必须加强临床基因扩增检验实验室的质量管理[2]。目前普遍采用Taq-...
A good linear range was obtained when 100–107 copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing ...
每个队列中，有10名受试者被随机分配接受研究药物或安慰剂（4:1）。使用经过验证的串联质谱方法(LC-MS/MS)来量化血浆中的 GST-HG141。药代动力学参数用WinNonlin 8.3软件计算。采用实时荧光定量PCR （QuantitativeReal-timePCR）来检测血清HBVDNA和HBV pgRNA，通过ELISA方法对血清HBV抗原进行测定。结果表明，使用25...
Real-time quantitative PCR After trypsin digestion, cells were harvested with low-speed centrifugation, and total RNA was extracted using the RNAeasy™ Animal RNA Isolation Kit (Beyotime, Cat#R0032). Subsequently, the Evo M-MLV RT Premix (AG, Cat# AG11706) was used to synthesize the compl...
荧光实时定量PCR（real-timequantitativePCR,RQ-PCR）技术在PCR反应体系中加入特异性的荧光染料或探针，荧光信号的变化真实地反映了体系中模板的增加，通过检测荧光信号，从而实时监测整个PCR反应过程，最后通过标准曲线对未知模板进行定量分析。HBVDNA定量检测 荧光定量PCR（fluorescencequantitativePCR,FQPCR），又称实时PCR，...
5.Real time quantitative PCR.Genome Res.6:986–994. 6.Kinetic PCR analysis: real-time monitoring of DNA amplification reaction.Bio/Technology11:1026–1030. 7.Williams JF.Optimization strategies for the polymerase chain ...
一个是定性(qualitative),一个是定量(quantitative) 。HBVDNA定性的注重于HBVDNA的存在,它通常可以测到低于100copies/ml的病毒存在,定性通常只给阴性、阳性的结果。定量的着重于HBV DNA量存在的多少。通常用于定量的极限在0.1-5 pg/ml之间。国外常用 fg/ml 为单位。copies/ml 比 fg/ml, pg/ml 单位更小。关于...
本规程适用于LightCycler荧光PCR检测仪检测乙型肝炎病毒（HBV-DNA）3试剂 中山大学达安基因公司乙肝病毒(HBV)核酸扩增荧光检测试剂盒 4仪器 Roche LightCycler荧光PCR检测仪 高速台式冷冻离心机 微量加样器（覆盖1-1000μl）5样品处理 样品处理按《PCR实验室标本处理规程》进行收集和处理，具体操作见本规程附录1和附录2。