hepatitis B virus PCR detection assay is vital for the evaluation of the hepatitis b development stage and efficacy of antiviral treatment. In this article, we discuss the importance of the HBV DNA quantitative PCR test in assessing HBV infectivity and prescribing antivirus treatment for hepatitis...
How to Perform Hepatitis B Virus DNA Quantitative Test? Accurate diagnosis is vital to control hepatitis B infection. Quantification Hepatitis B virus (HBV) DNA plays a critical role in the management of HBV infections. PCR-based assays are the most widely used method for HBV DNA quantification....
一个是定性(qualitative),一个是定量(quantitative) 。HBVDNA定性的注重于HBVDNA的存在,它通常可以测到低于100copies/ml的病毒存在,定性通常只给阴性、阳性的结果。定量的着重于HBV DNA量存在的多少。通常用于定量的极限在0.1-5 pg/ml之间。国外常用 fg/ml 为单位。copies/ml 比 fg/ml, pg/ml 单位更小。关于...
Key words:Real time fluorescence quantitative PCR; Polymerase chain reaction; HBV-DNA; Quality control HBV DNA含量是诊断和监测乙型病毒性肝炎的重要指标[1]。目前测定HBV DNA含量的常用方法是实时荧光定量PCR法,想获得可靠的HBV DNA含量测定结果,必须加强临床基因扩增检验实验室的质量管理[2]。目前普遍采用Taq-...
The HBV DNA digital PCR quantitative detection kit comprises PCR reaction primers SEQIDNo.1 and SEQIDNo.3 and a probe SEQIDNo.2; PCR reaction primers SEQIDNo.4 and SEQIDNo.6 and a probe SEQIDNo.5; PCR reaction primers SEQIDNo.7 and SEQIDNo.9 and a probe SEQIDNo.8; reference...
【主题词】 肝炎病毒,乙型;定量检测;性能验证;荧光定量PCRPerformance verification and evaluation of a one-step HBV DNA quantitative detecting systemYing耽i,Li Wanchun,Song Tao,Wang Jue,Mo Fugen,Wang Yipin,Cheng MaoliangCenter of Pathology and Laboratory Medicine,Sir RunRun Hospital,Na喝tng Medical ...
HBVDNA实时荧光定量检测程序的测量不确定度评估及分析 论著 临床研究 H B VＧD N A实时荧光定量检测程序的测量不确定度评估及分析∗ 刘伟平,杨新春,殷明刚ә (自贡市第一人民医院检验科,四川自贡６４３０００)㊀㊀摘㊀要:目的㊀根据国际标准化组织(I S O)１５１８９实验室认可要求,评估乙型肝炎病毒(H ...
乙型肝炎病毒核酸检测试剂盒(PCR-荧光法)cobas® HBV,蛋白酶溶液(PASE),DNA定量标准(DNA-QS),洗脱缓冲液(EB),主混合试剂1(MMX-R1),HBV主混合试剂2(HBV MMX-R2),乙型肝炎病毒核酸检测试剂盒(PCR-荧光法)cobas® HBV,<<me_syfw>。
探讨FQ—PCR检测HBV—DNA与CLEIA检测乙肝五项之间的相关性。方法 将以FQ—PCR法检测筛选的 HBV—DNA阳性结果的血清用CLEIA法对其进行乙肝病毒血清学标志物(即乙肝五项)的检测。结果 大三阳与小三阳 所占全部样本的比例最高,其低中高HBV—DNA水平的比例分别为17.2%,17.2%,65.5%和81.8%,15.2%,3.0%,而且 ...
乙型肝炎病毒荧光PCR检测试剂盒(HBV)是基于荧光染料检测的即用型 PCR 试剂盒,可以对靶片段进行实时定量PCR 分析(quantitative PCR、qPCR)。产品含有经过优化的缓冲液组分、Taq DNA聚合酶、dNTPs、MgCl2及新型荧光染料 DNAgreen 等所有实时定量 PCR 所需要的成分,用户只需加入模板和引物即可进行实时定量 PCR 反应和 HRM...