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To achieve overexpression of EGR1 or PFKL, the coding sequences (CDs) of EGR1 or PFKL were inserted into the MCS domain of the expression plasmid. Prof. Ni Tang (Key Laboratory of Molecular Biology on Infectious Diseases, Chongqing Medical University) supplied the LentiCRISPR-v2, pMD2.G, ...
We identified 1479 coding RNAs (mRNAs) and 232 long non-coding RNAs (lncRNAs) associated with METTL1 / WDR4 by using weighted coexpression network analysis (WGCNA) and co-clustering analysis. The least absolute shrinkage and selection operator (lasso) were used to constructing mRNA and lncRNA ...
The sequence coding for the intercellular domain of CD147 (residues 230–269 of CD147) was cloned into peGFP-C2 and dsRed2-C1 to produce eGFP-C2-ICD and dsRed2-C1- CD147-ICD, respectively. RQRNAAs of dsRed2-C1-CD147-ICD were deleted using a QuikChange Lightning Multi Site-Directed...
Table 1 Non-coding RNA in autophagy-related drug resistance of HCC. Full size table As an important participant in autophagy initiation, ULK1 has been reported to be associated with doxorubicin sensitivity. Furthermore, 30 clinical specimens were tested and the level of miR-26a/b in tumor tiss...
Nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver pathology worldwide. Meanwhile, liver cancer represents the sixth most common malignancy, with hepatocellular carcinoma (HCC) as the primary, most prevalent subtype. Due to the rising i
The simplest usage of -o is not to set any template arguments when downloading a single file, like in yt-dlp -o funny_video.flv "https://some/video" (hard-coding file extension like this is not recommended and could break some post-processing). It may however also contain special sequen...
Full size image To further confirm the findings from RNA-seq analysis, we examined the levels of cellular 2-NBDG, the indigestible glucose analog as a marker for glycolysis [21,22], in cells from a myriad of models in vitro. Notably, both CCHuh7R and CCPLC-PRF-5R displayed higher 2-...
As a set of evolutionarily conserved small non-coding RNAs, microRNAs (miRNAs) have been identified as post-transcriptional regulators at the level of gene expression, which is achieved by the combination of complementary sequences with the 3'-untranslated regions (UTRs) of corresponding mRNA of ...
Additionally, the long non-coding RNA HULC primarily regulates aberrant lipid metabolism in hepatoma cells through two mechanisms, thereby acting as an oncogene within these cells. On one hand, HULC enhances the expression of the transcriptional regulator PPARα, leading to the activation of the ...