实时荧光定量PCR仪器发展及现状 用实时荧光定量PCR检测石蜡标本中的麻风菌DNA以提高PB.ppt 实时荧光定量PCR技术原理 内源性ALV的全基因测序、JALV实时荧光定量PCR检测方法的建立及应用 学术论文:改良多重实时荧光PCR技术定量检测DNA甲基化(可编辑文本) 实时荧光定量PCR (realtime PCR) 技术原理(Roche公司 PPT) 实时荧...
377 Evaluation of the realquant® HBV-DNA assay (realtime PCR)We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10...
实时荧光定量PCR (realtime PCR) 技术原理(Roche公司 PPT) 热度: 【PPT】-HBVDNA定量检测 热度: JOURNALOFCLINICALMICROBIOLOGY,May2008,p.1716–1723Vol.46,No.5 0095-1137/08/$08.000doi:10.1128/JCM.01248-07 Copyright©2008,AmericanSocietyforMicrobiology.AllRightsReserved. ...
Real-time PCR quantification was performed using the QuantiFast Probe PCR kit in a RotorGene Q real-time PCR system (Qiagen, GmbH, Hilden, Germany) with the “over-gap” primers and dual hybridization probes with fluorescence resonance energy transfer technology, sequences of which have been ...
DNA was purified by standard phenol chloroform extraction protocol and assayed by quantitative PCR on a CFX96 real-time PCR system (Bio-Rad) or LC96 (Roche) with Talent qPCR PreMix (SYBR Green, TIANGEN). Fold enrichment was calculated as percentage input and normalized to total H3. (For ...
PCRassay,asatimesaving,laborsaving,andcheapmethodforquantifyingHBVDNAlevels,issuitableforapplicationinChina. 【Keywords】 DNA ,viral;hepatiti s B,chronic;reagent kits,diagnostic 全球约有 20 多亿人既往感染过 HBV,其 中约 3. 5亿 为慢性 HBV 感染者 fl】 ...
Fingerprick whole blood capillary samples were tested using the SD BIOLINE HCV POC-RDT, Determine HIV Early Detect and Determine HBsAg 2, and the results were assessed against the LIAISON XL HCV, HIV and Murex-HBsAg-Quant, reference assays, respectively. The feasibility and user satisfaction of ...
then used for HDV cDNA quantification:31,32forward (Kuo F: 5′-GGACCCCTTCAGCGAACA) and reverse (Kuo R: 5′-CCTAGCATCTCCTCCTATCGCTAT) primers. The qPCR was performed using FastStart Universal SYBR Green Master (Roche Applied Science) on a StepOne Real-Time PCR System (Applied Biosystems)...
The aim of this study was to develop an in-house real-time PCR-based method, which was both ultra-sensitive and efficient offering an alternative method for nucleic acid testing (NAT). Methods A precore fragment with 109bp was cloned and serial diluted to standard curve construction. The ...
The cDNAs were then used for real-time PCR (qPCR) on a CFX96 Touch Real-Time quantitative PCR System (Bio-Rad) using TB Green® Premix Ex Taq II (Tli RNaseH Plus; Takara). β-actin was served as the internal control. The relative quantification of gene expression was analyzed by ...